Detailed information of TA system TA139041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-relB/YoeB-YefM
• Location: 74648..75150
• Replicon: plasmid unnamed
• Accession: NZ_CP043574
• Organism: Comamonas testosteroni strain R5-28

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: Q5XQD8
• Locus tag: F0Q02_RS26690
• Protein ID: WP_032492620.1
• Coordinates: 74896..75150 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1V0BEH6
• Locus tag: F0Q02_RS26685
• Protein ID: WP_032492621.1
• Coordinates: 74648..74899 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0Q02_RS26655	70360..70641	+	282	WP_182312695.1	hypothetical protein	-
F0Q02_RS26660	70675..71379	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
F0Q02_RS26665	71556..72320	-	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
F0Q02_RS26670	72495..73334	-	840	WP_000259031.1	sulfonamide-resistant dihydropteroate synthase Sul1	-
F0Q02_RS26675	73328..73675	-	348	WP_000679427.1	quaternary ammonium compound efflux SMR transporter QacE delta 1	-
F0Q02_RS26680	73832..74464	-	633	WP_000186237.1	type B-3 chloramphenicol O-acetyltransferase CatB3	-
F0Q02_RS26685	74648..74899	+	252	WP_032492621.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
F0Q02_RS26690	74896..75150	+	255	WP_032492620.1	Txe/YoeB family addiction module toxin	Toxin
F0Q02_RS26695	75325..75879	-	555	WP_071846204.1	aminoglycoside 6'-N-acetyltransferase	-
F0Q02_RS26700	75962..76201	-	240	WP_165720489.1	DfrB family trimethoprim-resistant dihydrofolate reductase	-
F0Q02_RS26705	76462..77475	+	1014	WP_000845048.1	class 1 integron integrase IntI1	-
F0Q02_RS26710	77921..78148	+	228	WP_182312697.1	TraY domain-containing protein	-
F0Q02_RS26715	78132..78404	+	273	WP_174863007.1	type II toxin-antitoxin system RelE/ParE family toxin	-
F0Q02_RS26720	78437..78640	-	204	WP_174863008.1	hypothetical protein	-
F0Q02_RS26725	78631..79218	-	588	WP_182312700.1	cobyrinic acid ac-diamide synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / tet(G) / aph(6)-Id / aph(3'')-Ib / qacE / catB3 / aac(6')-IIc / dfrB7 / mph(A)	-	1..248727	248727
	-	inside	IScluster/Tn	sul1 / tet(G) / aph(6)-Id / aph(3'')-Ib / qacE / catB3 / aac(6')-IIc / dfrB7 / mph(A)	-	49794..90457	40663
	-	inside	Integron	-	-	73328..77475	4147

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10272.81 Da
• Isoelectric Point: 9.3585

>T139041 WP_032492620.1 NZ_CP043574:74896-75150 [Comamonas testosteroni]
MRLIFSDDAWEDYLFWQKQDRRMVDRINKLIKETTREPFSGVGKPEPLKHALAGYWSRRITDEHRMVYKVADDALWIVQL
KYHY

Nucleotide

• Length: 255 bp

>T139041 NZ_CP058342:2099319-2099422 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9244.40 Da
• Isoelectric Point: 4.7769

>AT139041 WP_032492621.1 NZ_CP043574:74648-74899 [Comamonas testosteroni]
MDAITYTHARANLAGTMDRVCNDHEPVIITRNGDQSVVILSLEDFQALEETAYLLRSPANAKRLFKSIEQLEAGRGQARE
LAE

Nucleotide

• Length: 252 bp

>AT139041 NZ_CP058342:c2099460-2099189 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A1V0BEH9

Antitoxin

• AlphaFold DB: A0A1V0BEH6