Detailed information of TA system TA139040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 5514357..5516047
• Replicon: chromosome
• Accession: NZ_CP043573
• Organism: Comamonas testosteroni strain R5-28

Toxin (Protein)

• Gene name: doc
• Uniprot ID: B7X3A3
• Locus tag: F0Q02_RS25230
• Protein ID: WP_003060410.1
• Coordinates: 5515169..5516047 (-)
• Length: 293 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: B7X3A4
• Locus tag: F0Q02_RS25225
• Protein ID: WP_003060412.1
• Coordinates: 5514357..5515163 (-)
• Length: 269 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0Q02_RS25205	5510367..5511143	-	777	WP_003060420.1	SDR family oxidoreductase	-
F0Q02_RS25210	5511458..5512216	-	759	WP_003060418.1	5'-methylthioadenosine/adenosylhomocysteine nucleosidase	-
F0Q02_RS25215	5512337..5513323	-	987	WP_003060416.1	tripartite tricarboxylate transporter substrate binding protein	-
F0Q02_RS25220	5513514..5514314	-	801	WP_003060414.1	aspartate dehydrogenase	-
F0Q02_RS25225	5514357..5515163	-	807	WP_003060412.1	IclR family transcriptional regulator	Antitoxin
F0Q02_RS25230	5515169..5516047	-	879	WP_003060410.1	alpha/beta hydrolase	Toxin
F0Q02_RS25235	5516056..5516589	-	534	WP_003060408.1	cupin domain-containing protein	-
F0Q02_RS25240	5516826..5518502	-	1677	WP_003060405.1	long-chain-fatty-acid--CoA ligase	-
F0Q02_RS25245	5518716..5519780	-	1065	WP_003060402.1	fructose-bisphosphate aldolase class II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (44-277)

Antitoxin

• Transcription regulator IclR, C-terminal (137-257)

Sequences

Toxin

Protein

• Length: 293 a.a.
• Molecular weight: 30487.93 Da
• Isoelectric Point: 6.3792

>T139040 WP_003060410.1 NZ_CP043573:c5516047-5515169 [Comamonas testosteroni]
MHEDLLSPKLQLLQTGFPERLVKLPQNAQVAWREAGDASSDFAVVLLHGISSGAASWLDVALQLGDKARVLAWDAPGYGV
STPLAPAAPADADYAQALAQSLLVLGVRRCLLVGHSLGALMAARLAVTAAPGLVEQLVFISPAGGYGAAAKAEQQAKVRE
GRLAALAEKGVAGLAAVIDQRLVSSEAPEAVRAWVRWNTARMQPQGYAQAVELLCGSDLAQAQGRLGMPVQVWVGEHDVV
TPPAACKAWSELLGAHYGTLAAAGHASPVEQPVEVAHRLASLLNQSYLPNTS

Nucleotide

• Length: 879 bp

>T139040 NZ_CP058342:c1875792-1875415 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 269 a.a.
• Molecular weight: 29237.21 Da
• Isoelectric Point: 5.0056

>AT139040 WP_003060412.1 NZ_CP043573:c5515163-5514357 [Comamonas testosteroni]
MSETPEISTNDQDRYTVPALERGLRLLACFGPEQPVWSAPELARNLELPRSTVFRMLTTLENSGYLQRSGTEYRLGLAVL
RLGYDYLSTQPLAQLAEPALQALCDELGMTSNLALRDGTSVVYVARVTPAGAFQGAVRVGSRLPAHATVLGRALLHDMDA
AQLRSVFGGEELPQFSESTPRNIDELLGLLAQDRERGYAMGEGFYEPGVSSIAAPVRAADGRVVAGLAMAIPFTELVPEK
TQLWVQKVQEAAEALSAHLRQQHPDHLR

Nucleotide

• Length: 807 bp

>AT139040 NZ_CP058342:c1876250-1875882 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCACCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: B7X3A3

Antitoxin

• AlphaFold DB: B7X3A4