Detailed information of TA system TA139038

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 59346..59938
• Replicon: plasmid unnamed
• Accession: NZ_CP043572
• Organism: Pseudomonas migulae strain R1-9

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: F0Q00_RS26620
• Protein ID: WP_182344856.1
• Coordinates: 59346..59648 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A6I1WJJ7
• Locus tag: F0Q00_RS26625
• Protein ID: WP_032901693.1
• Coordinates: 59645..59938 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0Q00_RS26585	54446..55285	+	840	WP_026014172.1	universal stress protein	-
F0Q00_RS26590	55285..55644	+	360	WP_003090764.1	TraR/DksA family transcriptional regulator	-
F0Q00_RS26595	55690..56652	+	963	WP_010794530.1	SdiA-regulated domain-containing protein	-
F0Q00_RS26600	56922..57167	-	246	WP_182344851.1	hypothetical protein	-
F0Q00_RS26605	57450..57767	-	318	WP_070414885.1	hypothetical protein	-
F0Q00_RS26610	57821..58279	-	459	WP_182344853.1	hypothetical protein	-
F0Q00_RS26615	58334..59167	-	834	WP_182344854.1	DUF945 domain-containing protein	-
F0Q00_RS26620	59346..59648	+	303	WP_182344856.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F0Q00_RS26625	59645..59938	+	294	WP_032901693.1	putative addiction module antidote protein	Antitoxin
F0Q00_RS26630	60711..60854	+	144	Protein_64	helix-turn-helix domain-containing protein	-
F0Q00_RS26635	61054..61605	-	552	WP_177031928.1	helix-turn-helix domain-containing protein	-
F0Q00_RS26640	62136..62759	-	624	WP_182344859.1	recombinase family protein	-
F0Q00_RS26645	62983..63333	-	351	WP_048377565.1	ParR	-
F0Q00_RS26650	63330..64238	-	909	WP_048377564.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	cmlA1 / aadA15 / qacE / floR / tet(G) / sul1 / aph(3'')-Ib / aph(6)-Id	-	1..68338	68338

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-89)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11294.00 Da
• Isoelectric Point: 9.5727

>T139038 WP_182344856.1 NZ_CP043572:59346-59648 [Pseudomonas migulae]
MKTIKQTATFMTWERKLKDHRAKAIIAARVFRVANGLLGDVEPVGQGVSELRIHYGPGYRVYFQQRGNELVLLLCGGDKS
SQARDIEIAKMLASQWSEDE

Nucleotide

• Length: 303 bp

>T139038 NZ_CP058342:1862484-1862735 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10535.04 Da
• Isoelectric Point: 5.6863

>AT139038 WP_032901693.1 NZ_CP043572:59645-59938 [Pseudomonas migulae]
MKEPIYEYDPAAALTDPESIAIFMSDAFETGDAGYIAKALGVVARAKGMTELAKESGLSREQLYRSFSDKGNPTLKTMLA
VMRAVGLDLTTRQHAHP

Nucleotide

• Length: 294 bp

>AT139038 NZ_CP058342:1862732-1862986 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A6I1WJJ7