Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4977322..4977947 Replicon chromosome
Accession NZ_CP043562
Organism Klebsiella pneumoniae strain P094-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag F0H50_RS24465 Protein ID WP_002882817.1
Coordinates 4977322..4977705 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag F0H50_RS24470 Protein ID WP_004150355.1
Coordinates 4977705..4977947 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F0H50_RS24450 4974688..4975590 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
F0H50_RS24455 4975587..4976222 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
F0H50_RS24460 4976219..4977148 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
F0H50_RS24465 4977322..4977705 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
F0H50_RS24470 4977705..4977947 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
F0H50_RS24475 4978152..4979069 + 918 WP_004178029.1 alpha/beta hydrolase -
F0H50_RS24480 4979083..4980024 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
F0H50_RS24485 4980069..4980506 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
F0H50_RS24490 4980503..4981363 - 861 WP_004146232.1 virulence factor BrkB family protein -
F0H50_RS24495 4981357..4981956 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T139030 WP_002882817.1 NZ_CP043562:c4977705-4977322 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T139030 NZ_CP058341:9951-10634 [Campylobacter coli]
ATGATTGATAAAGTTTTGAAATTTTTAGATGATGGTATTCCTAAAAATGATTTTGATAGACAACTTTTAGCTTCTAATTT
AGTAGGCGCAACAAATTTAATAGAGTTAAATAAAAAAGAAAGAATAATAACTCTACAAGCCGCTAAAAAATTAGAGCTTA
ATCCAATTAAAGGCAATTTTGACTATCAACACTTAAAAGATATACATAAAGCACTTTTTAAAGATGTTTATACTTGGGCT
GGAAAAGATAGAATGGAAGTAGGTTTGCATGGAAATTTTGGAAAATATGCACCAAATGGAACAATTACTAATTTTGTTCC
TGGAAAAGATTTAAATGTAACCGCTCAACAAATTTTTACTTGGCTTAAAGAAGACAATTACTTAAAAAATTCAAAAGATC
TTAACGATTTCGCAAAGAACTTAGCCGAATTTGCTAGAAATCTAAATGCTTTGCACCCCTTTAGAGAAGGTAACGGCAGA
ACTCAAAGAATATTTTTAAATGAGTTGGCTAAAAATGCAGGTTATAAACTTGATTTAAATTTAATACCAAAAGATAAAAC
GATAATGGCATCTGTAGAAGCTTCACAATTGAAATTAGGAAAACTTGAAGCCATAATTAAAACCAATCTAAAAAGCTTTA
GACAAAACTTAGATTTAGAGCAAAATAAAGGAATATCATTGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT139030 WP_004150355.1 NZ_CP043562:c4977947-4977705 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT139030 NZ_CP058341:9716-9958 [Campylobacter coli]
ATGCAAAAACAATATCCCGAAGTGCATAGCTTAGAAGAAAGCCTTGCGATACTTAAAAAATATAAAGATGATTTAACTAA
AGAGCAATATGAGCAAAATAAATCTATTATTTGTGGATTTGCTATTGAGAATATGTTTGCAAATGAAGAAGACATTATAA
ATCTCATAAAAGTAGATAAACAAGAAAAAACACCTGATGAAATAATCGCCGAATATAAAAAAGAGTGGGGTGTAAATGAT
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References