Detailed information of TA system TA139003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 1016691..1017201
• Replicon: chromosome
• Accession: NZ_CP043556
• Organism: Vibrio cholerae strain RFB16

Toxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0F2IA90
• Locus tag: F0316_RS18985
• Protein ID: WP_000098716.1
• Coordinates: 1016938..1017201 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: F0316_RS18980
• Protein ID: WP_149560835.1
• Coordinates: 1016691..1016945 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0316_RS19940	1011959..1012798	+	840	WP_142993905.1	hypothetical protein	-
F0316_RS18950	1013158..1013253	+	96	WP_001907607.1	DUF645 family protein	-
F0316_RS18955	1013459..1013998	+	540	WP_000884561.1	hydrolase	-
F0316_RS18960	1014235..1014831	+	597	WP_149560833.1	hypothetical protein	-
F0316_RS18965	1015154..1015333	+	180	WP_001898535.1	DUF645 family protein	-
F0316_RS18970	1015547..1015942	+	396	WP_044128232.1	DUF3465 domain-containing protein	-
F0316_RS18975	1016117..1016530	+	414	WP_172626089.1	hypothetical protein	-
F0316_RS18980	1016691..1016945	+	255	WP_149560835.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
F0316_RS18985	1016938..1017201	+	264	WP_000098716.1	Txe/YoeB family addiction module toxin	Toxin
F0316_RS18995	1017516..1017698	+	183	Protein_916	DUF645 family protein	-
F0316_RS19000	1018056..1018235	+	180	WP_001898535.1	DUF645 family protein	-
F0316_RS19005	1018440..1018664	+	225	WP_162797377.1	hypothetical protein	-
F0316_RS19010	1018936..1019343	+	408	WP_061058663.1	NUDIX hydrolase	-
F0316_RS19015	1019533..1019883	-	351	WP_076008713.1	hypothetical protein	-
F0316_RS19020	1019890..1021173	-	1284	WP_114725992.1	hypothetical protein	-
F0316_RS19030	1021697..1021879	+	183	WP_000947517.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	981561..1071623	90062

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10401.72 Da
• Isoelectric Point: 7.9816

>T139003 WP_000098716.1 NZ_CP043556:1016938-1017201 [Vibrio cholerae]
MSSRLLSWTDESWDDYLYWQTQDKKTLKRINKIINDVKRSPFEGIGKPEPLKENLSGFWSRRIDDTNRLVYAVDDNAITV
ISCRYHY

Nucleotide

• Length: 264 bp

>T139003 NZ_CP043556:1016938-1017201 [Vibrio cholerae]
ATGAGTAGTCGTTTACTGTCATGGACAGATGAGTCCTGGGATGACTACTTGTACTGGCAAACACAAGACAAAAAAACACT
CAAGCGGATCAATAAAATCATCAATGATGTTAAGCGTTCACCGTTTGAAGGCATTGGTAAACCAGAGCCTCTAAAGGAAA
ATCTATCTGGTTTTTGGTCTCGACGGATCGATGATACAAATAGACTGGTTTATGCTGTTGATGATAATGCAATAACGGTT
ATTTCGTGTCGCTACCATTATTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9449.70 Da
• Isoelectric Point: 5.0481

>AT139003 WP_149560835.1 NZ_CP043556:1016691-1016945 [Vibrio cholerae]
MRIVSFTEARNSLKAVLDAVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPANAEHLNRSIAQFKAGKVIQRD
LIDE

Nucleotide

• Length: 255 bp

>AT139003 NZ_CP058325:c4551144-4550851 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A0F2IA90