Detailed information of TA system TA139002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 1001108..1001874
• Replicon: chromosome
• Accession: NZ_CP043556
• Organism: Vibrio cholerae strain RFB16

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: F0316_RS18835
• Protein ID: WP_149560828.1
• Coordinates: 1001108..1001605 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: F0316_RS18840
• Protein ID: WP_000246254.1
• Coordinates: 1001602..1001874 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0316_RS18800	996493..996981	+	489	WP_000424240.1	hypothetical protein	-
F0316_RS19930	997127..997765	+	639	WP_172626087.1	hypothetical protein	-
F0316_RS18805	997901..998269	+	369	WP_057558163.1	hypothetical protein	-
F0316_RS18810	998403..998918	+	516	WP_000343786.1	GNAT family N-acetyltransferase	-
F0316_RS19935	999066..999170	+	105	WP_172626088.1	DUF3709 domain-containing protein	-
F0316_RS18820	999191..999424	+	234	WP_057562222.1	DUF3709 domain-containing protein	-
F0316_RS18825	999705..1000274	+	570	WP_000686204.1	flavodoxin family protein	-
F0316_RS18830	1000409..1000960	+	552	WP_000672156.1	hypothetical protein	-
F0316_RS18835	1001108..1001605	-	498	WP_149560828.1	GNAT family N-acetyltransferase	Toxin
F0316_RS18840	1001602..1001874	-	273	WP_000246254.1	DUF1778 domain-containing protein	Antitoxin
F0316_RS18855	1002795..1002974	+	180	WP_002021999.1	DUF645 family protein	-
F0316_RS18860	1003174..1004040	+	867	WP_149560829.1	hypothetical protein	-
F0316_RS18870	1004650..1005165	+	516	WP_001201533.1	lipocalin family protein	-
F0316_RS18875	1005323..1005859	+	537	WP_000469482.1	GNAT family N-acetyltransferase	-
F0316_RS18880	1006000..1006578	+	579	WP_000395682.1	O-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	981561..1071623	90062

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-89)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18493.18 Da
• Isoelectric Point: 8.7695

>T139002 WP_149560828.1 NZ_CP043556:c1001605-1001108 [Vibrio cholerae]
MMNTVLLDKDKHDKNRFNCGIEALNNYLKVIASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T139002 NZ_CP058325:c4417792-4417382 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9685.19 Da
• Isoelectric Point: 6.2988

>AT139002 WP_000246254.1 NZ_CP043556:c1001874-1001602 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLTKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVANAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT139002 NZ_CP058325:c4418039-4417773 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA