Detailed information of TA system TA138998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1223610..1224527
• Replicon: chromosome
• Accession: NZ_CP043546
• Organism: Bacillus velezensis strain SYP-B637

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: F0M21_RS06495
• Protein ID: WP_012117368.1
• Coordinates: 1223781..1224527 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: F0M21_RS06490
• Protein ID: WP_003154807.1
• Coordinates: 1223610..1223780 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0M21_RS06445	1218744..1219256	+	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-
F0M21_RS06450	1219369..1219590	+	222	Protein_1194	terminase	-
F0M21_RS06455	1219999..1220466	+	468	WP_077411063.1	hypothetical protein	-
F0M21_RS06460	1220479..1220850	+	372	WP_012117365.1	hypothetical protein	-
F0M21_RS06465	1220855..1221052	+	198	WP_012117366.1	XkdX family protein	-
F0M21_RS06470	1221109..1221870	+	762	WP_012117367.1	hypothetical protein	-
F0M21_RS06475	1221922..1222185	+	264	WP_003154815.1	hemolysin XhlA family protein	-
F0M21_RS06480	1222199..1222462	+	264	WP_003154813.1	phage holin	-
F0M21_RS06485	1222476..1223354	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
F0M21_RS06490	1223610..1223780	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
F0M21_RS06495	1223781..1224527	-	747	WP_012117368.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
F0M21_RS06500	1224632..1225630	-	999	WP_007407255.1	inorganic phosphate transporter	-
F0M21_RS06505	1225643..1226260	-	618	WP_003154804.1	DUF47 domain-containing protein	-
F0M21_RS06510	1226546..1227862	-	1317	WP_007610842.1	amino acid permease	-
F0M21_RS06515	1228185..1229135	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1211266..1281814	70548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29119.61 Da
• Isoelectric Point: 4.7003

>T138998 WP_012117368.1 NZ_CP043546:c1224527-1223781 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAEWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQADKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T138998 NZ_CP043546:c1224527-1223781 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGAATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAAGCGGATAAGGACCGCTTGACAGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTTCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT138998 WP_003154807.1 NZ_CP043546:c1223780-1223610 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT138998 NZ_CP043546:c1223780-1223610 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14