138997

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 488907..489544 Replicon chromosome
Accession NZ_CP043546
Organism Bacillus velezensis strain SYP-B637

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag F0M21_RS02525 Protein ID WP_003156187.1
Coordinates 489194..489544 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag F0M21_RS02520 Protein ID WP_003156188.1
Coordinates 488907..489188 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F0M21_RS02500 485272..485871 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
F0M21_RS02505 485964..486329 + 366 WP_012116869.1 holo-ACP synthase -
F0M21_RS02510 486494..487501 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
F0M21_RS02515 487618..488787 + 1170 WP_012116870.1 alanine racemase -
F0M21_RS02520 488907..489188 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
F0M21_RS02525 489194..489544 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
F0M21_RS02530 489662..490483 + 822 WP_003156182.1 STAS domain-containing protein -
F0M21_RS02535 490488..490853 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
F0M21_RS02540 490856..491257 + 402 WP_003156178.1 anti-sigma regulatory factor -
F0M21_RS02545 491269..492276 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
F0M21_RS02550 492340..492669 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
F0M21_RS02555 492666..493148 + 483 WP_007609591.1 anti-sigma B factor RsbW -
F0M21_RS02560 493114..493902 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
F0M21_RS02565 493902..494504 + 603 WP_012116871.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T138997 WP_003156187.1 NZ_CP043546:489194-489544 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T138997 NZ_CP058325:510594-510878 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT138997 WP_003156188.1 NZ_CP043546:488907-489188 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT138997 NZ_CP058325:510362-510604 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References