Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 84847..86470 Replicon plasmid pF2_18C_HI2
Accession NZ_CP043545
Organism Escherichia coli strain F2_81

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag CPH39_RS25390 Protein ID WP_000088046.1
Coordinates 85145..86470 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag CPH39_RS25385 Protein ID WP_024136327.1
Coordinates 84847..85140 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPH39_RS26720 80790..80921 + 132 WP_000374059.1 hypothetical protein -
CPH39_RS25360 (CPH39_25855) 81262..81492 - 231 WP_065311109.1 hypothetical protein -
CPH39_RS25370 (CPH39_25865) 82854..83045 - 192 WP_000398480.1 hypothetical protein -
CPH39_RS25375 (CPH39_25870) 83137..83478 - 342 WP_001151575.1 hypothetical protein -
CPH39_RS25380 (CPH39_25875) 83851..84243 + 393 WP_000341066.1 hypothetical protein -
CPH39_RS25385 (CPH39_25885) 84847..85140 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
CPH39_RS25390 (CPH39_25890) 85145..86470 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
CPH39_RS25395 (CPH39_25895) 86531..86737 + 207 WP_042634301.1 hypothetical protein -
CPH39_RS25400 (CPH39_25900) 86839..87129 + 291 WP_000985910.1 hypothetical protein -
CPH39_RS25405 (CPH39_25910) 87171..88007 - 837 WP_000480968.1 aminoglycoside O-phosphotransferase APH(6)-Id -
CPH39_RS25410 (CPH39_25915) 88007..88810 - 804 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib -
CPH39_RS25415 (CPH39_25920) 88917..89848 - 932 Protein_87 IS3-like element IS1133 family transposase -
CPH39_RS25420 (CPH39_25925) 89881..90849 + 969 WP_000654804.1 IS5-like element IS903B family transposase -
CPH39_RS25425 (CPH39_25930) 90906..91112 - 207 Protein_89 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(6)-Id / aph(3'')-Ib / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / tet(A) / dfrA12 / qacE / sul1 / aph(3')-IIa - 1..274059 274059
- inside IScluster/Tn aph(6)-Id / aph(3'')-Ib / sul3 / ant(3'')-Ia / cmlA1 / aadA2 - 81523..110032 28509


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T138995 WP_000088046.1 NZ_CP043545:85145-86470 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T138995 NZ_CP058322:5839573-5839983 [Micromonospora carbonacea]
GTGATCTACCTCGATTCCGCCGCCGTCGTCAAGTTGGTCCGGCAGGAGGCGTGCAGTGCCGACCTTGTCTCCTGGCTCAA
CGCTCACGACGACGTGCCGCTGGTCTCCTCCGCACTCGTCGAGGTGGAAGTGCCCAGAGCGTTACGCCGGTCGGCGCCGC
AAGCCTTGATCGGCGTGCCGGCGGCAGTCGGACGGCTCTTCCGACTGGAGATCGACAGCACGATCCGCGCAACCGCAGCC
GCGTTCGATGAGCCGAACCTGCGTAGCCTCGACGCCATCCACCTGGCCACCGCCCAGCTTCTGACCAACGAGTCCGGAAC
GGCACTCATCGCCTTCGTCACCTACGACCGACGACTGCTCGAATGCGCCAAGGAAGCAGGACTGCCCGTAGCGAGCCCCG
GCCAGAACTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT138995 WP_024136327.1 NZ_CP043545:84847-85140 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT138995 NZ_CP058322:5839298-5839576 [Micromonospora carbonacea]
ATGGAGCGCATCGGTGTCCGGGAGCTGAACCAGAACACGAGCCAGGTGCTGGCCCGGGTGAGCGGCGGCGAGACCGTCGA
GATCACCGACCGCGGGCATCCGATCGCCCGGCTTGTTCCAGTTGGTGACGACCGGTCGATGCTAGCCAGGCTGGTGGCTG
CGGGGCGGGCCGTCGCCCCGACCGGCGGCGGTCCTGTTCCGCTTCCACCGCAACTCGGTGACGAGGATGTGGACGTAGCC
GCCACGCTCGCCGCGATGCGGGACGAGGAGCGCTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References