Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 46281..46828 Replicon plasmid pF2_18C_FIB
Accession NZ_CP043544
Organism Escherichia coli strain F2_81

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag CPH39_RS24795 Protein ID WP_001384452.1
Coordinates 46550..46828 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID Q4FIL6
Locus tag CPH39_RS24790 Protein ID WP_000079945.1
Coordinates 46281..46550 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPH39_RS24750 (CPH39_25165) 41658..42374 + 717 WP_101981710.1 A24 family peptidase -
CPH39_RS24755 (CPH39_25170) 42468..42869 + 402 WP_001222322.1 GspS family T2SS pilot lipoprotein variant EptO -
CPH39_RS24760 (CPH39_25175) 42995..43138 + 144 Protein_41 IS3 family transposase -
CPH39_RS24765 (CPH39_25185) 43451..43744 + 294 Protein_42 hypothetical protein -
CPH39_RS24770 (CPH39_25190) 43973..44221 + 249 WP_044864812.1 replication regulatory protein RepA -
CPH39_RS24775 (CPH39_25200) 44466..44540 + 75 WP_001365705.1 RepA leader peptide Tap -
CPH39_RS24780 (CPH39_25205) 44533..45366 + 834 WP_033809053.1 incFII family plasmid replication initiator RepA -
CPH39_RS24785 46068..46226 + 159 WP_000841008.1 hypothetical protein -
CPH39_RS24790 (CPH39_25220) 46281..46550 + 270 WP_000079945.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
CPH39_RS24795 (CPH39_25225) 46550..46828 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
CPH39_RS24800 (CPH39_25230) 47064..48251 - 1188 WP_096220698.1 IS91 family transposase -
CPH39_RS24805 (CPH39_25235) 48436..49649 + 1214 WP_220541910.1 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - stcE / exeE / exeG / hlyD / hlyB / hlyA / hlyC 1..82910 82910


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T138994 WP_001384452.1 NZ_CP043544:46550-46828 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T138994 NZ_CP058322:5838833-5839207 [Micromonospora carbonacea]
ATGACCCTGCTGCTGGACACGCACGTCGCGCTCTGGGCCATCACCGGTGATGCGACCCTCGGCGTCGATTTCCTCGACCA
GCTTCGCCACGACCCAGACATCCTTCTCTCCCCCGTCACCTTGTGGGAGATCACCATCAAGCAGGGCGCGGGAAAGCTCG
TCGGGCCGCCTGACCTCGCCGAGCGGGTAAGGGAGATGGGCTTCCGTGAGCTCCCGGTGACCCATGCCCACGCCATCGCC
GCCAGCCGCCTGCCGCCCCACCACCGCGACCCCTTCGACCGCATGCTGGTGGCGCAGGCGATCACCGAGGGTCTGACGCT
GGCCACCCGCGACGCATCGATAGCGCTGTACGACGTGGACGTCCTCAAGGTGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10180.52 Da        Isoelectric Point: 8.8735

>AT138994 WP_000079945.1 NZ_CP043544:46281-46550 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLGRGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT138994 NZ_CP058322:5839298-5839576 [Micromonospora carbonacea]
ATGGAGCGCATCGGTGTCCGGGAGCTGAACCAGAACACGAGCCAGGTGCTGGCCCGGGTGAGCGGCGGCGAGACCGTCGA
GATCACCGACCGCGGGCATCCGATCGCCCGGCTTGTTCCAGTTGGTGACGACCGGTCGATGCTAGCCAGGCTGGTGGCTG
CGGGGCGGGCCGTCGCCCCGACCGGCGGCGGTCCTGTTCCGCTTCCACCGCAACTCGGTGACGAGGATGTGGACGTAGCC
GCCACGCTCGCCGCGATGCGGGACGAGGAGCGCTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB Q4FIL6

References