Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4188827..4189377 Replicon chromosome
Accession NZ_CP043542
Organism Escherichia coli strain F2_81

Toxin (Protein)


Gene name ccdB Uniprot ID D3H432
Locus tag CPH39_RS20250 Protein ID WP_001160975.1
Coordinates 4188827..4189141 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID -
Locus tag CPH39_RS20255 Protein ID WP_000125567.1
Coordinates 4189144..4189377 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPH39_RS20225 (4184502) 4184502..4185788 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
CPH39_RS20230 (4185788) 4185788..4186777 + 990 WP_096220317.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
CPH39_RS20235 (4186774) 4186774..4187595 + 822 WP_096220318.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
CPH39_RS20240 (4187598) 4187598..4187975 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
CPH39_RS20245 (4187982) 4187982..4188830 + 849 WP_000257188.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
CPH39_RS20250 (4188827) 4188827..4189141 - 315 WP_001160975.1 CcdB family protein Toxin
CPH39_RS20255 (4189144) 4189144..4189377 - 234 WP_000125567.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
CPH39_RS20260 (4189463) 4189463..4189942 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
CPH39_RS20265 (4190134) 4190134..4191996 - 1863 WP_000377117.1 glutathione-regulated potassium-efflux system protein KefC -
CPH39_RS20270 (4191989) 4191989..4192519 - 531 WP_000600718.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
CPH39_RS20275 (4192630) 4192630..4193961 - 1332 WP_001183198.1 MFS transporter -
CPH39_RS20280 (4194020) 4194020..4194307 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.76 Da        Isoelectric Point: 9.8517

>T138992 WP_001160975.1 NZ_CP043542:c4189141-4188827 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T138992 NZ_CP058321:c2759101-2758823 [Halomonas sp. SH5A2]
ATGATCAAGAGCTTTCGACACAAAGGCCTGCGTAAGTTCTTCGAAACCGGCTCAACTTCCGGTATTCAGGCCAAGCACGC
GACCAAGTTGCAAATCCAGCTAACCGCGCTGGACAGTGCAAAGGGCCCTGAGGATATGAATGCGCCGGGTTGGAGGCTGC
ATCCTTTGTCCCACAAGCTGAAAGGACACTGGTCTATCACTGTCAACAGGAACTGGCGACTTACCTTCGCTTTCGAAGGT
GAGGATGCCATCCTCGTTGATTATCAAGACTATCACTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8544.55 Da        Isoelectric Point: 5.8147

>AT138992 WP_000125567.1 NZ_CP043542:c4189377-4189144 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSEEYRSF

Download         Length: 234 bp

>AT138992 NZ_CP058321:c2758814-2758533 [Halomonas sp. SH5A2]
ATGAATCGCATGCATAACCCCGCACATCCTGGCGCTGTTTTGCGCGAGTACATCGGGGATATCTCTGTTACCCAGGCGGC
CCAGCAGCTTGGCGTGACGCGTACTGCGCTATCGCGGATTCTGAACGGTAATGCCGGTATTTCAGCTGACATGGCACTGA
GACTTGAGCAAGCATTGGGTACCAGCGCCGAAATGTGGCTGGAAATGCAGCTCAAGTATGAGCTATGGCAAGCGTCACAG
CGTCCACGCGCTCAAGTCTCTCCTCTTCACGCGGCAGGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References