Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3694327..3694945 Replicon chromosome
Accession NZ_CP043542
Organism Escherichia coli strain F2_81

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CPH39_RS17920 Protein ID WP_001291435.1
Coordinates 3694727..3694945 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CPH39_RS17915 Protein ID WP_000344800.1
Coordinates 3694327..3694701 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPH39_RS17905 (3689416) 3689416..3690609 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CPH39_RS17910 (3690632) 3690632..3693781 + 3150 WP_053883285.1 efflux RND transporter permease AcrB -
CPH39_RS17915 (3694327) 3694327..3694701 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CPH39_RS17920 (3694727) 3694727..3694945 + 219 WP_001291435.1 HHA domain-containing protein Toxin
CPH39_RS17925 (3695117) 3695117..3695668 + 552 WP_096220366.1 maltose O-acetyltransferase -
CPH39_RS17930 (3695784) 3695784..3696254 + 471 WP_000136192.1 YlaC family protein -
CPH39_RS17935 (3696418) 3696418..3697968 + 1551 WP_053883318.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CPH39_RS17940 (3698010) 3698010..3698363 - 354 WP_000878140.1 DUF1428 domain-containing protein -
CPH39_RS17950 (3698742) 3698742..3699053 + 312 WP_000409911.1 MGMT family protein -
CPH39_RS17955 (3699084) 3699084..3699656 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T138989 WP_001291435.1 NZ_CP043542:3694727-3694945 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T138989 NZ_CP058321:963353-963664 [Halomonas sp. SH5A2]
ATGTTATTCATCGAAACCTCAGCGTTTACCAAGCTGTTACCGAACTACCTCACGGATGAAGAATACCGTGGGCTGCAAGT
GTATCTGCTGCAGAAACCGGATGCGGGCGACCTCATCAAAGGTTCGGGTGGTGTTCGTAAGGTTCGCTGGTCATCAGCAG
GTTCGGGAAAGAGCGGTGGGGTCAGGGCTATCTATTATTGGAAAAAATCTGACCACGAGATATGGATGCTCACGCTGTAC
AGTAAAACCGAGCGTGCCAGTATTCCTGGGCACATGCTCAAGCAAATAGCGGAGGCCATCAAAAATGGTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT138989 WP_000344800.1 NZ_CP043542:3694327-3694701 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT138989 NZ_CP058321:963657-963944 [Halomonas sp. SH5A2]
ATGGTTGATAGAAATCTAGGAGCAGAGATCCTCGACGGAATTGATGAAATTAAGCAATTCAAAAAAGGCAACGTCGCGCT
GCGAACTCACGAGTTAGCCGAGCCTTCACCCCCTAAAATCATTCGTCATAAACTGAATTTATCGCAATCCGCCTTTGCGG
GCCTTCTTGGGGTGAGCATGCGTACTTTGCAGGATTGGGAGCAAGGGCGAAGAGAGCCACAAGGCCCTGCCGTTGCCCTT
TTGCGTATTGCTGAGCAACACCCTGAAGTCTTTGATCAGCTGCATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References