Detailed information of TA system TA138987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2518185..2518823
• Replicon: chromosome
• Accession: NZ_CP043542
• Organism: Escherichia coli strain F2_81

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: CPH39_RS12235
• Protein ID: WP_000813794.1
• Coordinates: 2518647..2518823 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CPH39_RS12230
• Protein ID: WP_001270286.1
• Coordinates: 2518185..2518601 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPH39_RS12210 (2513337)	2513337..2514278	-	942	WP_053883752.1	ABC transporter permease	-
CPH39_RS12215 (2514279)	2514279..2515292	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
CPH39_RS12220 (2515310)	2515310..2516455	-	1146	WP_089637768.1	ABC transporter substrate-binding protein	-
CPH39_RS12225 (2516697)	2516697..2518106	-	1410	WP_096220621.1	PLP-dependent aminotransferase family protein	-
CPH39_RS12230 (2518185)	2518185..2518601	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CPH39_RS12235 (2518647)	2518647..2518823	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CPH39_RS12240 (2519045)	2519045..2519275	+	231	WP_000494244.1	YncJ family protein	-
CPH39_RS12245 (2519367)	2519367..2521328	-	1962	WP_001515270.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CPH39_RS12250 (2521401)	2521401..2521937	-	537	WP_000429150.1	DNA-binding transcriptional regulator SutR	-
CPH39_RS12255 (2521990)	2521990..2523204	+	1215	WP_072169390.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T138987 WP_000813794.1 NZ_CP043542:c2518823-2518647 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T138987 NZ_CP058321:704573-704875 [Halomonas sp. SH5A2]
ATGGCTGAAGTAATCTGGACTGAGCCGGCCCTTCAAGAATTAGACGCAATCGCAGAGTACATTGCTCTGGATAATCCTGC
CGCTGCAAGCCGCCTGGTAGAAGAGATTTTCGATAAAACCGACCGCTTGGAAGATTTTCCCCATTCCGGAAGGATTCCTC
CAGAGTTCTCTAATTCGGTATACAGGGAGGTAGTGGTTCCACCGTGTCGTATTTTTTATCGTGAGGCCGAGAAGCAGGTT
CTCGTCCTCTATGTCATGCGAGAGGAGCGGCAGCTTCGTGCGTACATGCTCGGGAACAGCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT138987 WP_001270286.1 NZ_CP043542:c2518601-2518185 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT138987 NZ_CP058321:704328-704585 [Halomonas sp. SH5A2]
ATGAAAGTTGAGCTTGTTACAAACCTTAAGCGCCAAGCCACAAAAATCTTGGCAGACCTGCATCTGTCCAAAGAGCCGGT
GCTGATCACTGAACATGGCCAGCCATCCGCTTATCTTGTGGATGTGCAGGATTACGAGTTCATGCAGCGTCGGCTTGAGC
TGCTCGAGGGGCTCTCCCGGGGTGAGCGTGCTGTACTTGAGGGAAGAACGTACAGCCAAAGTGAGGCCAGGGAGAAAATG
AGTAAATGGCTGAAGTAA

Structures

Toxin

• PDB: 6HPB