Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 896527..897181 Replicon chromosome
Accession NZ_CP043542
Organism Escherichia coli strain F2_81

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag CPH39_RS04310 Protein ID WP_000244777.1
Coordinates 896774..897181 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CPH39_RS04305 Protein ID WP_000354046.1
Coordinates 896527..896793 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPH39_RS04285 (892497) 892497..893930 - 1434 WP_053883955.1 6-phospho-beta-glucosidase BglA -
CPH39_RS04290 (893975) 893975..894286 + 312 WP_062892423.1 N(4)-acetylcytidine aminohydrolase -
CPH39_RS04295 (894450) 894450..895109 + 660 WP_053883911.1 hemolysin III family protein -
CPH39_RS04300 (895304) 895304..896284 - 981 WP_053883913.1 tRNA-modifying protein YgfZ -
CPH39_RS04305 (896527) 896527..896793 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CPH39_RS04310 (896774) 896774..897181 + 408 WP_000244777.1 protein YgfX Toxin
CPH39_RS04315 (897221) 897221..897742 - 522 WP_001055874.1 flavodoxin FldB -
CPH39_RS04320 (897854) 897854..898750 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CPH39_RS04325 (898775) 898775..899485 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CPH39_RS04330 (899491) 899491..901224 + 1734 WP_000813168.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T138972 WP_000244777.1 NZ_CP043542:896774-897181 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T138972 NZ_CP058312:866840-867016 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTCATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT138972 WP_000354046.1 NZ_CP043542:896527-896793 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT138972 NZ_CP043542:896527-896793 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References