Detailed information of TA system TA138971

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 700202..701034
• Replicon: chromosome
• Accession: NZ_CP043542
• Organism: Escherichia coli strain F2_81

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: F4TJC5
• Locus tag: CPH39_RS03340
• Protein ID: WP_001094452.1
• Coordinates: 700660..701034 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CPH39_RS03335
• Protein ID: WP_220541894.1
• Coordinates: 700202..700570 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPH39_RS03315 (697958)	697958..698775	+	818	Protein_652	DUF945 domain-containing protein	-
CPH39_RS03320 (698867)	698867..699352	+	486	WP_000206663.1	antirestriction protein	-
CPH39_RS03325 (699368)	699368..699844	+	477	WP_001186706.1	RadC family protein	-
CPH39_RS03330 (699907)	699907..700128	+	222	WP_000692345.1	DUF987 domain-containing protein	-
CPH39_RS03335 (700202)	700202..700570	+	369	WP_220541894.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
CPH39_RS03340 (700660)	700660..701034	+	375	WP_001094452.1	TA system toxin CbtA family protein	Toxin
CPH39_RS03345 (701031)	701031..701522	+	492	WP_130069251.1	DUF5983 family protein	-
CPH39_RS03350 (701534)	701534..701731	+	198	WP_000772035.1	DUF957 domain-containing protein	-
CPH39_RS03355 (701828)	701828..702673	+	846	WP_001290261.1	DUF4942 domain-containing protein	-
CPH39_RS03365 (702972)	702972..703478	+	507	WP_000228941.1	G/U mismatch-specific DNA glycosylase	-
CPH39_RS03370 (703583)	703583..704911	+	1329	WP_053884740.1	IS4-like element IS4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	665001..720214	55213
	-	flank	IS/Tn	-	-	703583..704911	1328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14014.86 Da
• Isoelectric Point: 7.2920

>T138971 WP_001094452.1 NZ_CP043542:700660-701034 [Escherichia coli]
MNTLPDTHVREASRCSSPVTIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSTDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T138971 NZ_CP043542:700660-701034 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCTCATCTCCCGTAACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTAAATGACACACCGTTCTCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCACCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13537.40 Da
• Isoelectric Point: 6.6249

>AT138971 WP_220541894.1 NZ_CP043542:700202-700570 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSCGYVYLAVYPTSETKK

Nucleotide

• Length: 369 bp

>AT138971 NZ_CP058311:c94665-94372 [Escherichia coli]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGCTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGCGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCATCCTGGGCGTAAAAATCAGCTTCTCCTCGGCAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGGATTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7Z7YIM6