Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 308100..308900 Replicon chromosome
Accession NZ_CP043542
Organism Escherichia coli strain F2_81

Toxin (Protein)


Gene name ataT Uniprot ID A0A0K4AFE4
Locus tag CPH39_RS01360 Protein ID WP_001614005.1
Coordinates 308373..308900 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A8S7FBC9
Locus tag CPH39_RS01355 Protein ID WP_053883614.1
Coordinates 308100..308366 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPH39_RS01335 (303758) 303758..304426 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
CPH39_RS01340 (304419) 304419..305477 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
CPH39_RS01345 (305722) 305722..306576 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
CPH39_RS01350 (306847) 306847..307950 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
CPH39_RS01355 (308100) 308100..308366 + 267 WP_053883614.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
CPH39_RS01360 (308373) 308373..308900 + 528 WP_001614005.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
CPH39_RS01365 (308897) 308897..309280 - 384 WP_000778770.1 aspartate 1-decarboxylase autocleavage activator PanM -
CPH39_RS01370 (309704) 309704..310813 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
CPH39_RS01375 (310861) 310861..311787 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
CPH39_RS01380 (311784) 311784..313061 + 1278 WP_001626130.1 branched chain amino acid ABC transporter permease LivM -
CPH39_RS01385 (313058) 313058..313825 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19676.62 Da        Isoelectric Point: 7.3502

>T138968 WP_001614005.1 NZ_CP043542:308373-308900 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T138968 NZ_CP058310:c12357-12151 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9940.40 Da        Isoelectric Point: 10.0863

>AT138968 WP_053883614.1 NZ_CP043542:308100-308366 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWMRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT138968 NZ_CP058310:12344-12405 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4AFE4

Antitoxin

Source ID Structure

References