Detailed information of TA system TA138959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PsyrTA/-
• Location: 3596860..3599188
• Replicon: chromosome
• Accession: NZ_CP043539
• Organism: Escherichia coli O157 strain Al Ain

Toxin (Protein)

• Gene name: PsyrT
• Uniprot ID: A0A6N0MHC8
• Locus tag: F0L19_RS17945
• Protein ID: WP_000532146.1
• Coordinates: 3596860..3597834 (+)
• Length: 325 a.a.

Antitoxin (Protein)

• Gene name: PsyrA
• Uniprot ID: -
• Locus tag: F0L19_RS17950
• Protein ID: WP_001303704.1
• Coordinates: 3597893..3599188 (+)
• Length: 432 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0L19_RS17920	3592717..3593712	+	996	WP_000730263.1	gluconate operon transcriptional repressor GntR	-
F0L19_RS17925	3593851..3594378	+	528	WP_032164465.1	gluconokinase	-
F0L19_RS17930	3594382..3595722	+	1341	WP_000210111.1	gluconate transporter	-
F0L19_RS17935	3595862..3596470	+	609	WP_000410808.1	ATP-dependent DNA helicase	-
F0L19_RS17940	3596455..3596856	+	402	WP_000502498.1	ATP-dependent DNA helicase	-
F0L19_RS17945	3596860..3597834	+	975	WP_000532146.1	ATP-dependent DNA helicase	Toxin
F0L19_RS17950	3597893..3599188	+	1296	WP_001303704.1	DNA-protecting protein DprA	Antitoxin
F0L19_RS17955	3599234..3599827	-	594	WP_001002544.1	NAAT family transporter YhgN	-
F0L19_RS17960	3600019..3601122	+	1104	WP_000799956.1	aspartate-semialdehyde dehydrogenase	-
F0L19_RS17965	3601395..3603581	+	2187	WP_001283734.1	1,4-alpha-glucan branching enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• DNA recombination-mediator protein A (115-429)

Sequences

Toxin

Protein

• Length: 325 a.a.
• Molecular weight: 36460.93 Da
• Isoelectric Point: 9.2667

>T138959 WP_000532146.1 NZ_CP043539:3596860-3597834 [Escherichia coli O157]
VGRAGRGIDSAVGILLCGSEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQRTNLRYGQIEKALKLLVAENP
SPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELADVESYITTKECKMQFLRRALDEPGAEHCGKCSSCLQHPLLSPDI
DSGLLHAANLFIKHADLSLNLNKQVAAGAFTQYGFKGNLPASLQGSTGRILSRWGYSGWGKQVAQEKKTGRFSDELVEAC
AEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFESTSTWFLILPGDWRRNLAYLLLMPLKKSWTIHRRKCSKTVSTSVKIS
TGRL

Nucleotide

• Length: 975 bp

>T138959 NZ_CP058308:2754470-2754577 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 432 a.a.
• Molecular weight: 47053.95 Da
• Isoelectric Point: 7.2590

>AT138959 WP_001303704.1 NZ_CP043539:3597893-3599188 [Escherichia coli O157]
MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLLTSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAE
LLVPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLI
AGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENG
ASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESISIERLASCT
GLTIEQISVWLNRAEEEGRVIRLGEGHYQFR

Nucleotide

• Length: 1296 bp

>AT138959 NZ_CP058308:c2754417-2754356 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A6N0MHC8