Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2711090..2711587 Replicon chromosome
Accession NZ_CP043539
Organism Escherichia coli O157 strain Al Ain

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag F0L19_RS13700 Protein ID WP_001173343.1
Coordinates 2711090..2711263 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag F0L19_RS13705 Protein ID WP_000398619.1
Coordinates 2711291..2711587 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F0L19_RS13690 2707760..2709217 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
F0L19_RS13695 2709454..2710971 + 1518 WP_001295074.1 lysine--tRNA ligase -
F0L19_RS13700 2711090..2711263 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
F0L19_RS13705 2711291..2711587 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
F0L19_RS13710 2711814..2712086 - 273 WP_000405647.1 N-acetyltransferase -
F0L19_RS13715 2712098..2712328 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
F0L19_RS13720 2712509..2714140 + 1632 WP_001216477.1 sensor histidine kinase -
F0L19_RS13725 2714137..2714856 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T138955 WP_001173343.1 NZ_CP043539:c2711263-2711090 [Escherichia coli O157]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T138955 NZ_CP043539:c2711263-2711090 [Escherichia coli O157]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT138955 WP_000398619.1 NZ_CP043539:c2711587-2711291 [Escherichia coli O157]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT138955 NZ_CP058308:c2096404-2096133 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References