Detailed information of TA system TA138954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 2614099..2614694
• Replicon: chromosome
• Accession: NZ_CP043539
• Organism: Escherichia coli O157 strain Al Ain

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: F0L19_RS13195
• Protein ID: WP_000239579.1
• Coordinates: 2614099..2614449 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: F0L19_RS13200
• Protein ID: WP_001223210.1
• Coordinates: 2614443..2614694 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0L19_RS13175	2609545..2610567	-	1023	WP_001301928.1	ABC transporter permease	-
F0L19_RS13180	2610581..2612083	-	1503	WP_000205806.1	sugar ABC transporter ATP-binding protein	-
F0L19_RS13185	2612223..2613179	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
F0L19_RS13190	2613489..2614019	+	531	WP_000055075.1	inorganic diphosphatase	-
F0L19_RS13195	2614099..2614449	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
F0L19_RS13200	2614443..2614694	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
F0L19_RS13205	2614906..2615247	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
F0L19_RS13210	2615250..2619029	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T138954 WP_000239579.1 NZ_CP043539:c2614449-2614099 [Escherichia coli O157]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T138954 NZ_CP058308:c1909060-1908686 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCTGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT138954 WP_001223210.1 NZ_CP043539:c2614694-2614443 [Escherichia coli O157]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT138954 NZ_CP058308:c1909454-1909149 [Escherichia coli]
CTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGC
CGGTATCAGAGGCCTGTTCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAAC
TCATGCTCACCAGCGGTGAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCC
GATACCCTCAGCAGTTGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8