Detailed information of TA system TA138951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2375101..2375651
• Replicon: chromosome
• Accession: NZ_CP043539
• Organism: Escherichia coli O157 strain Al Ain

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: F0L19_RS12195
• Protein ID: WP_001160975.1
• Coordinates: 2375101..2375415 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: F0L19_RS12200
• Protein ID: WP_000125566.1
• Coordinates: 2375418..2375651 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0L19_RS12170	2370776..2372062	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
F0L19_RS12175	2372062..2373051	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
F0L19_RS12180	2373048..2373869	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
F0L19_RS12185	2373872..2374249	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
F0L19_RS12190	2374256..2375104	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
F0L19_RS12195	2375101..2375415	-	315	WP_001160975.1	CcdB family protein	Toxin
F0L19_RS12200	2375418..2375651	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
F0L19_RS12205	2375737..2376216	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
F0L19_RS12210	2376408..2378270	-	1863	WP_021500082.1	glutathione-regulated potassium-efflux system protein KefC	-
F0L19_RS12215	2378263..2378793	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
F0L19_RS12220	2378901..2380232	-	1332	WP_001183177.1	MFS transporter	-
F0L19_RS12225	2380290..2380577	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T138951 WP_001160975.1 NZ_CP043539:c2375415-2375101 [Escherichia coli O157]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T138951 NZ_CP058308:1366968-1367366 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT138951 WP_000125566.1 NZ_CP043539:c2375651-2375418 [Escherichia coli O157]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT138951 NZ_CP058308:1366741-1366968 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9