Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 6237995..6238578 Replicon chromosome
Accession NZ_CP043538
Organism Methylobacterium mesophilicum SR1.6/6

Toxin (Protein)


Gene name PumA Uniprot ID A0A6B9FUH5
Locus tag MMSR116_RS29790 Protein ID WP_010684550.1
Coordinates 6238276..6238578 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A6B9FSV4
Locus tag MMSR116_RS29785 Protein ID WP_010684549.1
Coordinates 6237995..6238276 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMSR116_RS29745 6233256..6233666 + 411 WP_010684542.1 phage major tail protein, TP901-1 family -
MMSR116_RS29750 6233684..6234016 + 333 WP_010684543.1 gene transfer agent family protein -
MMSR116_RS29755 6233992..6234237 + 246 WP_083920253.1 phage tail assembly chaperone -
MMSR116_RS29760 6234246..6234818 + 573 WP_010684544.1 phage tail tape measure protein -
MMSR116_RS29765 6234844..6235755 - 912 WP_010684545.1 TauD/TfdA family dioxygenase -
MMSR116_RS29770 6236007..6236648 + 642 WP_010684546.1 DUF2460 domain-containing protein -
MMSR116_RS29775 6236659..6237534 + 876 WP_010684547.1 DUF2163 domain-containing protein -
MMSR116_RS29780 6237531..6238061 + 531 WP_010684548.1 C40 family peptidase -
MMSR116_RS29785 6237995..6238276 - 282 WP_010684549.1 putative addiction module antidote protein Antitoxin
MMSR116_RS29790 6238276..6238578 - 303 WP_010684550.1 hypothetical protein Toxin
MMSR116_RS29795 6238658..6242551 + 3894 WP_010684551.1 glycoside hydrolase/phage tail family protein -
MMSR116_RS29800 6242568..6243536 + 969 Protein_5941 DUF2793 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 6224111..6247305 23194


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(4-96)

Antitoxin

Uncharacterized protein HI1420

(3-89)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 10895.55 Da        Isoelectric Point: 10.5803

>T138930 WP_010684550.1 NZ_CP043538:c6238578-6238276 [Methylobacterium mesophilicum SR1.6/6]
VTTILLSDTFRTWPAGLKDHRAKARIAAQIRAAGFDNVGNVKPVGSGVCEMRIPYGPGYRVYYVRRGEVTSLLLCGGDKA
SQTRDIQRAKRMAANLDGTV

Download         Length: 303 bp

>T138930 NZ_CP058302:c4389455-4389348 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 9978.43 Da        Isoelectric Point: 4.5881

>AT138930 WP_010684549.1 NZ_CP043538:c6238276-6237995 [Methylobacterium mesophilicum SR1.6/6]
MRFTPFDASEFLDSEAAIAEYLAAALDDPDPELFIAALGDVAKARGMSQIAREAGVGRESLYKALTPGSKLRYETVRKVM
DVLGVKLTVSPAS

Download         Length: 282 bp

>AT138930 NZ_CP058302:4389502-4389568 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6B9FUH5

Antitoxin

Source ID Structure
AlphaFold DB A0A6B9FSV4

References