Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 82206..82942 Replicon plasmid unnamed4
Accession NZ_CP043515
Organism Enterobacter kobei strain EB_P8_L5_01.19

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag FZO55_RS25245 Protein ID WP_003026803.1
Coordinates 82206..82688 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag FZO55_RS25250 Protein ID WP_003026799.1
Coordinates 82676..82942 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZO55_RS25225 78564..78815 + 252 Protein_73 recombinase family protein -
FZO55_RS25230 78963..79943 - 981 WP_149410393.1 IS5-like element ISKpn26 family transposase -
FZO55_RS25235 80032..80580 + 549 Protein_75 abortive infection family protein -
FZO55_RS25240 80651..81994 - 1344 WP_080339306.1 ISNCY family transposase -
FZO55_RS25245 82206..82688 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
FZO55_RS25250 82676..82942 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
FZO55_RS25260 83387..83617 - 231 WP_023307222.1 hypothetical protein -
FZO55_RS25265 83631..83834 - 204 WP_004213596.1 hemolysin expression modulator Hha -
FZO55_RS25280 84079..85047 + 969 WP_172971211.1 IS5 family transposase -
FZO55_RS25285 85411..86151 + 741 WP_001515717.1 tyrosine-type recombinase/integrase -
FZO55_RS25290 86849..87859 - 1011 WP_000200070.1 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..125849 125849
- inside IScluster/Tn - - 73181..85047 11866


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T138925 WP_003026803.1 NZ_CP043515:c82688-82206 [Enterobacter kobei]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T138925 NZ_CP058302:c3537941-3537723 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT138925 WP_003026799.1 NZ_CP043515:c82942-82676 [Enterobacter kobei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT138925 NZ_CP043515:c82942-82676 [Enterobacter kobei]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References