Detailed information of TA system TA138924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 141..16413
• Replicon: plasmid unnamed3
• Accession: NZ_CP043514
• Organism: Enterobacter kobei strain EB_P8_L5_01.19

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A155IU92
• Locus tag: FZO55_RS24750
• Protein ID: WP_000754567.1
• Coordinates: 141..557 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: FZO55_RS24745
• Protein ID: WP_149410388.1
• Coordinates: 16413..144 (+)
• Length: -5422.6666666667 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZO55_RS24750	141..557	+	417	WP_000754567.1	type II toxin-antitoxin system VapC family toxin	Toxin
FZO55_RS24755	908..1875	-	968	Protein_2	IS5 family transposase	-
FZO55_RS24760	1903..3702	-	1800	Protein_3	Tn3 family transposase	-
FZO55_RS24770	3730..4434	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
FZO55_RS24775	4498..4755	-	258	Protein_5	transposase	-
FZO55_RS24780	4829..5467	+	639	Protein_6	IS5-like element IS903B family transposase	-
FZO55_RS24785	5532..5663	-	132	Protein_7	IS1 family transposase	-
FZO55_RS24790	5837..7057	+	1221	WP_000343760.1	ISL3-like element ISKox3 family transposase	-
FZO55_RS24795	7123..7554	+	432	WP_006796638.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
FZO55_RS24800	7554..8816	+	1263	WP_032656724.1	Y-family DNA polymerase	-
FZO55_RS24805	8898..9872	-	975	WP_000064119.1	ParB/RepB/Spo0J family partition protein	-
FZO55_RS24810	9872..11077	-	1206	WP_006797591.1	AAA family ATPase	-
FZO55_RS24815	11414..11680	+	267	Protein_13	hypothetical protein	-
FZO55_RS24825	12074..12940	-	867	WP_023302476.1	replication initiation protein	-
FZO55_RS26135	13475..13588	+	114	WP_032427600.1	hypothetical protein	-
FZO55_RS24830	13717..13974	+	258	WP_000764642.1	hypothetical protein	-
FZO55_RS24835	14032..14820	-	789	WP_022631000.1	site-specific integrase	-
FZO55_RS26140	14840..15511	-	672	WP_024140048.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..16427	16427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15108.61 Da
• Isoelectric Point: 8.5403

>T138924 WP_000754567.1 NZ_CP043514:141-557 [Enterobacter kobei]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T138924 NZ_CP058302:3318904-3319302 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: -5422.6666666667 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT138924 WP_149410388.1 NZ_CP043514:16413-144 [Enterobacter kobei]

Nucleotide

• Length: -16268 bp

>AT138924 NZ_CP058302:3318608-3318901 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A155IU92