Detailed information of TA system TA138910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1771081..1771671
• Replicon: chromosome
• Accession: NZ_CP043511
• Organism: Enterobacter kobei strain EB_P8_L5_01.19

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A8B2Y1G2
• Locus tag: FZO55_RS08570
• Protein ID: WP_045134957.1
• Coordinates: 1771339..1771671 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: FZO55_RS08565
• Protein ID: WP_058690818.1
• Coordinates: 1771081..1771338 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZO55_RS08530	1766149..1766553	+	405	WP_197739253.1	hypothetical protein	-
FZO55_RS08535	1766546..1767736	+	1191	WP_058690946.1	MFS transporter	-
FZO55_RS08540	1767785..1768654	+	870	WP_058690945.1	cation:proton antiporter	-
FZO55_RS08545	1768755..1769734	-	980	Protein_1631	IS5-like element ISKpn26 family transposase	-
FZO55_RS08555	1770525..1770715	+	191	Protein_1632	helix-turn-helix domain-containing protein	-
FZO55_RS08565	1771081..1771338	+	258	WP_058690818.1	hypothetical protein	Antitoxin
FZO55_RS08570	1771339..1771671	+	333	WP_045134957.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FZO55_RS08580	1771966..1774155	-	2190	WP_058690819.1	TonB-dependent siderophore receptor	-
FZO55_RS08585	1774182..1775507	-	1326	WP_058690820.1	SidA/IucD/PvdA family monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11785.63 Da
• Isoelectric Point: 10.5834

>T138910 WP_045134957.1 NZ_CP043511:1771339-1771671 [Enterobacter kobei]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLDGAGTKTMGAIRCDQPRTI
DMAARSGKRLERIPDAVVNEVLARLDAILN

Nucleotide

• Length: 333 bp

>T138910 NZ_CP058289:227404-227793 [Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9455.96 Da
• Isoelectric Point: 4.4488

>AT138910 WP_058690818.1 NZ_CP043511:1771081-1771338 [Enterobacter kobei]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPVV
GKEIL

Nucleotide

• Length: 258 bp

>AT138910 NZ_CP043511:1771081-1771338 [Enterobacter kobei]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATAAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAACTGCTTGCACAGTGCGATCCGCACGCCGAAATGAGCGACGAGGATCGGGAATGGATTGATGCGCCTGTGGTG
GGCAAGGAGATCCTGTAA

Structures

Toxin

• AlphaFold DB: A0A8B2Y1G2