Detailed information of TA system TA138893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1300802..1301343
• Replicon: chromosome
• Accession: NZ_CP043498
• Organism: Rhizobium grahamii strain BG7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FZ934_RS06505
• Protein ID: WP_153270398.1
• Coordinates: 1301020..1301343 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FZ934_RS06500
• Protein ID: WP_153270397.1
• Coordinates: 1300802..1301023 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZ934_RS06480	1296495..1297379	+	885	WP_153270394.1	alpha/beta hydrolase	-
FZ934_RS06485	1297537..1298307	+	771	WP_153270395.1	triose-phosphate isomerase	-
FZ934_RS06490	1298415..1298963	+	549	WP_113362982.1	preprotein translocase subunit SecG	-
FZ934_RS06495	1299104..1300732	+	1629	WP_153270396.1	CTP synthase	-
FZ934_RS06500	1300802..1301023	+	222	WP_153270397.1	antitoxin MazE family protein	Antitoxin
FZ934_RS06505	1301020..1301343	+	324	WP_153270398.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FZ934_RS06510	1301454..1302542	-	1089	WP_153270399.1	citrate synthase/methylcitrate synthase	-
FZ934_RS06515	1302627..1303771	+	1145	Protein_1282	citrate synthase	-
FZ934_RS06520	1303869..1304396	+	528	WP_153270400.1	FecR domain-containing protein	-
FZ934_RS06525	1304403..1306261	+	1859	Protein_1284	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (7-70)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11525.42 Da
• Isoelectric Point: 8.9352

>T138893 WP_153270398.1 NZ_CP043498:1301020-1301343 [Rhizobium grahamii]
MRRGDLVTVALSGDFGKPRPALVIQSDSFDATETVTLVLISGTLVDAPLLRITIHPDSGNGLQKPSQIMIDKTMTVRRER
IGQHVGRLGDEAMISVTRSLALFLGIA

Nucleotide

• Length: 324 bp

>T138893 NZ_CP058253:c3840182-3839772 [Enterobacter roggenkampii]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGCCTGGTTGCGGC
CTTTGTGTTACTGATGCCGTGGCCGCTGAGCTATACGCCGCTGTGGCTGCTGCTACTTTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCCCGTCAGGGAGAGATCAAGTTACTGATGGATTCCCGCCTGCGCTGGCAGGGAAAA
GAGTGGGATATTCTCGGTATGCCCTGGATGCTCAACTGCGGCATGATGTTACGGCTACGCAAGGTGGACGGCGGGCGTTG
CCAGCATCTTTGGCTGGCGGCTGACAGTATGGATGCTGCCGAGTGGCGAGACCTGCGCCGGATGCTATTGCAACAGACGA
CGCAGGGGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8137.24 Da
• Isoelectric Point: 6.9795

>AT138893 WP_153270397.1 NZ_CP043498:1300802-1301023 [Rhizobium grahamii]
MQSDVKARVKKRRDSLRASGLRPVQIWVPDTRRPGFLEECRRQSAVVAKADAADVGLHPFMDAAIADMDDADE

Nucleotide

• Length: 222 bp

>AT138893 NZ_CP058253:c3840429-3840163 [Enterobacter roggenkampii]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACACCTTAAGCGACGACGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA