Detailed information of TA system TA138889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 5916130..5916700
• Replicon: chromosome
• Accession: NZ_CP043497
• Organism: Streptomyces rimosus subsp. rimosus strain SRI-4018v0

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: L8ERH5
• Locus tag: FMM49_RS27250
• Protein ID: WP_003982453.1
• Coordinates: 5916353..5916700 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: L8ERT3
• Locus tag: FMM49_RS27245
• Protein ID: WP_003982452.1
• Coordinates: 5916130..5916366 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FMM49_RS27230 (FMM49_27355)	5911552..5912709	+	1158	WP_003982449.1	allantoicase	-
FMM49_RS27235 (FMM49_27360)	5912781..5915357	-	2577	WP_003982450.1	aminopeptidase N	-
FMM49_RS27240 (FMM49_27365)	5915491..5916090	+	600	WP_003982451.1	Uma2 family endonuclease	-
FMM49_RS27245 (FMM49_27370)	5916130..5916366	+	237	WP_003982452.1	ribbon-helix-helix domain-containing protein	Antitoxin
FMM49_RS27250 (FMM49_27375)	5916353..5916700	+	348	WP_003982453.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FMM49_RS27255 (FMM49_27380)	5916781..5917302	+	522	WP_003982454.1	DUF1203 domain-containing protein	-
FMM49_RS27260 (FMM49_27385)	5917373..5918404	-	1032	WP_003982455.1	aspartate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12493.43 Da
• Isoelectric Point: 10.8691

>T138889 WP_003982453.1 NZ_CP043497:5916353-5916700 [Streptomyces rimosus subsp. rimosus]
MRRGDLYLVDLEPVRGSEANKYRPAVVVSNDAANRSVQRAGRGVVTVVPVTANVARVYPFQVLLTADDCGLPRDSKAQCE
QVRAVSVERLGRRVGSVPARVMVGLDAALRRHLAL

Nucleotide

• Length: 348 bp

>T138889 NZ_CP058253:587808-588095 [Enterobacter roggenkampii]
ATGCCAACGGAGTATGAGTGGGATAGCAATAAGGCGAAAAGTAACCTGCAAAAACATGGCATACGCTTTGAGGATGCGGT
TATGGTTTTCGACGATCCTTATCATTTGTCTGTTCAGGATCGCTATGAAAACGGGGAATTTCGCTGGCAAACCATCGGCC
TTGTGCAAGGTCTTCTTGTTATTCTGGTCGCACATACGGTACGTTTTGAAAGCGGAGGAGAAATCATCCGTATCATTAGC
GCACGAAAGGCGGACCGCAAAGAGAGGAGCCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8374.07 Da
• Isoelectric Point: 3.7856

>AT138889 WP_003982452.1 NZ_CP043497:5916130-5916366 [Streptomyces rimosus subsp. rimosus]
MKISVSLPEEDIAFLDAYGKKTDADSRSAVIHAAVELLRASELEADYASAWEEWDESDDALLWDGVSGDGIGSADAAR

Nucleotide

• Length: 237 bp

>AT138889 NZ_CP043497:5916130-5916366 [Streptomyces rimosus subsp. rimosus]
ATGAAGATCAGCGTCAGCCTGCCGGAAGAGGACATCGCCTTCCTCGACGCGTACGGCAAGAAGACGGACGCCGATTCGCG
GTCGGCGGTGATCCACGCGGCGGTCGAGCTGCTGCGTGCCTCCGAGCTGGAGGCGGATTACGCCTCGGCGTGGGAGGAGT
GGGACGAGAGCGACGACGCGCTGCTGTGGGACGGGGTCTCCGGGGACGGGATCGGAAGCGCCGATGCGGCGCGGTGA

Structures

Toxin

• AlphaFold DB: L8ERH5

Antitoxin

• AlphaFold DB: L8ERT3