Detailed information of TA system TA138866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1568344..1568982
• Replicon: chromosome
• Accession: NZ_CP043487
• Organism: Escherichia coli strain AQ15

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: FQT78_RS07525
• Protein ID: WP_000813794.1
• Coordinates: 1568344..1568520 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FQT78_RS07530
• Protein ID: WP_001270285.1
• Coordinates: 1568566..1568982 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQT78_RS07505	1563963..1565177	-	1215	WP_001326689.1	BenE family transporter YdcO	-
FQT78_RS07510	1565230..1565766	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
FQT78_RS07515	1565839..1567800	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
FQT78_RS07520	1567892..1568122	-	231	WP_000494244.1	YncJ family protein	-
FQT78_RS07525	1568344..1568520	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
FQT78_RS07530	1568566..1568982	+	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
FQT78_RS07535	1569061..1570467	+	1407	WP_000760593.1	PLP-dependent aminotransferase family protein	-
FQT78_RS07540	1570712..1571857	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
FQT78_RS07545	1571875..1572888	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
FQT78_RS07550	1572889..1573830	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1562775..1563923	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T138866 WP_000813794.1 NZ_CP043487:1568344-1568520 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T138866 NZ_CP058236:c3158322-3157930 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT138866 WP_001270285.1 NZ_CP043487:1568566-1568982 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT138866 NZ_CP058236:c3158534-3158319 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• PDB: 6HPB