Detailed information of TA system TA138857

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4663165..4663872
• Replicon: chromosome
• Accession: NZ_CP043486
• Organism: Escherichia coli strain SL112

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: E0J2C8
• Locus tag: FQT94_RS22445
• Protein ID: WP_000691790.1
• Coordinates: 4663531..4663872 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FQT94_RS22440
• Protein ID: WP_000939438.1
• Coordinates: 4663165..4663500 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQT94_RS22415	4659112..4659330	-	219	WP_001064742.1	AlpA family phage regulatory protein	-
FQT94_RS22420	4659448..4660062	-	615	WP_000772909.1	inovirus Gp2 family protein	-
FQT94_RS22425	4660655..4661608	+	954	WP_000290405.1	hypothetical protein	-
FQT94_RS22430	4661844..4662662	+	819	WP_000761991.1	DUF945 domain-containing protein	-
FQT94_RS22435	4662692..4663165	+	474	WP_001292742.1	DNA repair protein RadC	-
FQT94_RS22440	4663165..4663500	+	336	WP_000939438.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FQT94_RS22445	4663531..4663872	+	342	WP_000691790.1	TA system toxin CbtA family protein	Toxin
FQT94_RS22450	4663987..4664820	+	834	WP_001192746.1	DUF4942 domain-containing protein	-
FQT94_RS22455	4664890..4665285	+	396	WP_000152741.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
FQT94_RS22460	4665278..4666225	+	948	WP_000342500.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
FQT94_RS22465	4666618..4667883	+	1266	WP_001218329.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	4647573..4696566	48993

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13060.08 Da
• Isoelectric Point: 9.4583

>T138857 WP_000691790.1 NZ_CP043486:4663531-4663872 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRCYNTTAR

Nucleotide

• Length: 342 bp

>T138857 NZ_CP058236:2890843-2891256 [Mycobacterium tuberculosis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12639.63 Da
• Isoelectric Point: 7.7723

>AT138857 WP_000939438.1 NZ_CP043486:4663165-4663500 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPWQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT138857 NZ_CP058236:2891295-2891552 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGACTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: E0J2C8