Detailed information of TA system TA138853

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3927614..3928333
• Replicon: chromosome
• Accession: NZ_CP043486
• Organism: Escherichia coli strain SL112

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: E0J373
• Locus tag: FQT94_RS18960
• Protein ID: WP_001095907.1
• Coordinates: 3928013..3928333 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E0J374
• Locus tag: FQT94_RS18955
• Protein ID: WP_001192122.1
• Coordinates: 3927614..3927979 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQT94_RS18920	3922708..3922998	+	291	Protein_3706	hypothetical protein	-
FQT94_RS18930	3924423..3925226	+	804	WP_001245321.1	hypothetical protein	-
FQT94_RS18935	3925443..3925919	+	477	WP_001254693.1	hypothetical protein	-
FQT94_RS18940	3925991..3926812	+	822	WP_000197395.1	DUF945 domain-containing protein	-
FQT94_RS18945	3926882..3927352	+	471	WP_000104319.1	DNA repair protein RadC	-
FQT94_RS18950	3927361..3927582	+	222	WP_000691985.1	DUF987 family protein	-
FQT94_RS18955	3927614..3927979	+	366	WP_001192122.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FQT94_RS18960	3928013..3928333	+	321	WP_001095907.1	TA system toxin CbtA family protein	Toxin
FQT94_RS18970	3929100..3930284	+	1185	WP_001172876.1	sugar efflux transporter	-
FQT94_RS18975	3930395..3931318	+	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-
FQT94_RS18980	3931322..3931585	-	264	Protein_3717	lipoprotein NlpA	-
FQT94_RS18985	3931600..3932808	-	1209	WP_001339197.1	IS4-like element ISVsa5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3911686..3932808	21122
	-	flank	IS/Tn	-	-	3931600..3932808	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (5-104)

Antitoxin

• Antitoxin CbeA (18-116)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12184.76 Da
• Isoelectric Point: 5.5740

>T138853 WP_001095907.1 NZ_CP043486:3928013-3928333 [Escherichia coli]
MNTPTVPATVTVSSRLSPVQVWQKLLTYILEHHYGLTINDTPFSNDTTIQEHIEAGVNLTDAVNFLVERFELVRIDQKGF
SWQDQEPWITSLDVRRAQFNLGLKRS

Nucleotide

• Length: 321 bp

>T138853 NZ_CP058236:c2393018-2392584 [Mycobacterium tuberculosis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13470.22 Da
• Isoelectric Point: 6.8421

>AT138853 WP_001192122.1 NZ_CP043486:3927614-3927979 [Escherichia coli]
MQSTTLSGTSAENVSCLQWGLKRNITPCFGARLVQEGNRLYFLADRAGFNGSFSDDEALRLDQAFPLMMKQLERMLTAGE
LDPRSQHCVTRHHNGLTCEADTLGSHGYVYIAIYPQSALTR

Nucleotide

• Length: 366 bp

>AT138853 NZ_CP058236:c2393279-2393025 [Mycobacterium tuberculosis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Structures

Toxin

• AlphaFold DB: E0J373

Antitoxin

• AlphaFold DB: E0J374