Detailed information of TA system TA138852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3663795..3664595
• Replicon: chromosome
• Accession: NZ_CP043486
• Organism: Escherichia coli strain SL112

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4NNI0
• Locus tag: FQT94_RS17765
• Protein ID: WP_000342449.1
• Coordinates: 3663795..3664322 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: FQT94_RS17770
• Protein ID: WP_001277108.1
• Coordinates: 3664329..3664595 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQT94_RS17740	3658870..3659637	-	768	WP_000082110.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
FQT94_RS17745	3659634..3660911	-	1278	WP_000803798.1	branched chain amino acid ABC transporter permease LivM	-
FQT94_RS17750	3660908..3661834	-	927	WP_000003004.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
FQT94_RS17755	3661882..3662991	-	1110	WP_000827696.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
FQT94_RS17760	3663415..3663798	+	384	WP_000778781.1	aspartate 1-decarboxylase autocleavage activator PanM	-
FQT94_RS17765	3663795..3664322	-	528	WP_000342449.1	GNAT family N-acetyltransferase	Toxin
FQT94_RS17770	3664329..3664595	-	267	WP_001277108.1	DUF1778 domain-containing protein	Antitoxin
FQT94_RS17775	3664745..3665848	-	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
FQT94_RS17780	3666119..3666973	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
FQT94_RS17785	3667218..3668276	-	1059	WP_001042003.1	permease-like cell division protein FtsX	-
FQT94_RS17790	3668269..3668937	-	669	WP_000617723.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19691.70 Da
• Isoelectric Point: 7.7457

>T138852 WP_000342449.1 NZ_CP043486:c3664322-3663795 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T138852 NZ_CP058236:2347629-2348039 [Mycobacterium tuberculosis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT138852 WP_001277108.1 NZ_CP043486:c3664595-3664329 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT138852 NZ_CP058236:2347403-2347636 [Mycobacterium tuberculosis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A829CLZ4

Antitoxin

• AlphaFold DB: A0A829CN24