Detailed information of TA system TA138838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1714834..1715360
• Replicon: chromosome
• Accession: NZ_CP043486
• Organism: Escherichia coli strain SL112

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: FQT94_RS08225
• Protein ID: WP_000323025.1
• Coordinates: 1714834..1715121 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: FQT94_RS08230
• Protein ID: WP_000534858.1
• Coordinates: 1715121..1715360 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQT94_RS08180	1709858..1710073	-	216	WP_000839590.1	phage lysis protein EssD	-
FQT94_RS08185	1710827..1711042	-	216	WP_000066484.1	cold shock-like protein CspB	-
FQT94_RS08190	1711343..1711555	+	213	WP_000087756.1	cold shock-like protein CspF	-
FQT94_RS08195	1711610..1711699	+	90	WP_120795389.1	hypothetical protein	-
FQT94_RS08200	1711977..1712729	-	753	WP_001047135.1	antitermination protein	-
FQT94_RS08205	1712743..1713792	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
FQT94_RS08210	1713794..1714072	-	279	WP_012304870.1	hypothetical protein	-
FQT94_RS08215	1714139..1714390	-	252	WP_000980994.1	hypothetical protein	-
FQT94_RS08220	1714607..1714762	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
FQT94_RS08225	1714834..1715121	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
FQT94_RS08230	1715121..1715360	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
FQT94_RS08235	1715385..1715690	+	306	WP_001326990.1	hypothetical protein	-
FQT94_RS08240	1715893..1716225	+	333	WP_001301033.1	protein FlxA	-
FQT94_RS08245	1716662..1717975	-	1314	WP_000589005.1	ISNCY family transposase	-
FQT94_RS23105	1718744..1719031	-	288	Protein_1619	hypothetical protein	-
FQT94_RS08255	1719642..1719998	-	357	WP_001310834.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1678096..1729861	51765

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T138838 WP_000323025.1 NZ_CP043486:c1715121-1714834 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T138838 NZ_CP058236:c1394696-1394403 [Mycobacterium tuberculosis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT138838 WP_000534858.1 NZ_CP043486:c1715360-1715121 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT138838 NZ_CP058236:c1394962-1394693 [Mycobacterium tuberculosis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8