Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 31762..32369 Replicon plasmid pDMEA1
Accession NZ_CP043485
Organism Enterococcus faecium strain DMEA02

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag FZP47_RS12870 Protein ID WP_002329852.1
Coordinates 32019..32369 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag FZP47_RS12865 Protein ID WP_002329851.1
Coordinates 31762..32025 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZP47_RS12835 27431..28471 + 1041 WP_149484509.1 replication initiator protein A -
FZP47_RS12845 28571..29741 + 1171 Protein_26 IS256 family transposase -
FZP47_RS12850 29850..30186 - 337 Protein_27 recombinase family protein -
FZP47_RS12855 30394..30591 - 198 WP_002329849.1 hypothetical protein -
FZP47_RS12860 30612..31124 - 513 WP_149484510.1 hypothetical protein -
FZP47_RS12865 31762..32025 + 264 WP_002329851.1 hypothetical protein Antitoxin
FZP47_RS12870 32019..32369 + 351 WP_002329852.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FZP47_RS12875 32393..32527 - 135 Protein_32 helix-turn-helix domain-containing protein -
FZP47_RS12880 32579..33163 - 585 WP_149484511.1 recombinase family protein -
FZP47_RS12885 33404..33597 + 194 Protein_34 AbrB family transcriptional regulator -
FZP47_RS12890 33663..33788 + 126 Protein_35 type II toxin-antitoxin system PemK/MazF family toxin -
FZP47_RS12895 33847..35178 - 1332 WP_002329854.1 helix-turn-helix domain-containing protein -
FZP47_RS13735 35879..36037 - 159 WP_167818090.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ClpL bopD 1..168626 168626
- inside IScluster/Tn - - 28571..33163 4592


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13282.35 Da        Isoelectric Point: 9.3335

>T138828 WP_002329852.1 NZ_CP043485:32019-32369 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGRRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSKYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T138828 NZ_CP058236:c758066-757683 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10169.58 Da        Isoelectric Point: 4.6865

>AT138828 WP_002329851.1 NZ_CP043485:31762-32025 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAGNLENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT138828 NZ_CP058236:c758316-758161 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References