Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3999810..4000464 Replicon chromosome
Accession NZ_CP043478
Organism Escherichia coli strain ST130

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag FZ043_RS19925 Protein ID WP_000244772.1
Coordinates 3999810..4000217 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FZ043_RS19930 Protein ID WP_000354046.1
Coordinates 4000198..4000464 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZ043_RS19905 3995767..3997500 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
FZ043_RS19910 3997506..3998216 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FZ043_RS19915 3998241..3999137 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FZ043_RS19920 3999249..3999770 + 522 WP_001055874.1 flavodoxin FldB -
FZ043_RS19925 3999810..4000217 - 408 WP_000244772.1 protein YgfX Toxin
FZ043_RS19930 4000198..4000464 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FZ043_RS19935 4000707..4001687 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FZ043_RS19940 4001764..4002423 - 660 WP_000250274.1 hemolysin III family protein -
FZ043_RS19945 4002587..4002898 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
FZ043_RS19950 4002943..4004376 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
FZ043_RS19955 4004433..4005176 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T138822 WP_000244772.1 NZ_CP043478:c4000217-3999810 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T138822 NZ_CP058236:c699391-698999 [Mycobacterium tuberculosis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT138822 WP_000354046.1 NZ_CP043478:c4000464-4000198 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT138822 NZ_CP058236:c699645-699388 [Mycobacterium tuberculosis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References