Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3862213..3862796 Replicon chromosome
Accession NZ_CP043478
Organism Escherichia coli strain ST130

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag FZ043_RS19270 Protein ID WP_000254745.1
Coordinates 3862213..3862548 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag FZ043_RS19275 Protein ID WP_000581937.1
Coordinates 3862548..3862796 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZ043_RS19250 3857682..3858044 + 363 WP_000034929.1 hypothetical protein -
FZ043_RS19255 3858100..3859398 - 1299 WP_000036723.1 phosphopyruvate hydratase -
FZ043_RS19260 3859486..3861123 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
FZ043_RS19265 3861351..3862142 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
FZ043_RS19270 3862213..3862548 - 336 WP_000254745.1 endoribonuclease MazF Toxin
FZ043_RS19275 3862548..3862796 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
FZ043_RS19280 3862874..3865108 - 2235 WP_000226815.1 GTP pyrophosphokinase -
FZ043_RS19285 3865156..3866457 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T138821 WP_000254745.1 NZ_CP043478:c3862548-3862213 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T138821 NZ_CP058236:682081-682488 [Mycobacterium tuberculosis]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGTCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT138821 WP_000581937.1 NZ_CP043478:c3862796-3862548 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT138821 NZ_CP058236:681869-682084 [Mycobacterium tuberculosis]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References