Detailed information of TA system TA138820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3550004..3550629
• Replicon: chromosome
• Accession: NZ_CP043478
• Organism: Escherichia coli strain ST130

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FZ043_RS17740
• Protein ID: WP_000911330.1
• Coordinates: 3550004..3550402 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: FZ043_RS17745
• Protein ID: WP_000450524.1
• Coordinates: 3550402..3550629 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZ043_RS17720	3545882..3546082	+	201	WP_000383836.1	YpfN family protein	-
FZ043_RS17725	3546192..3546890	-	699	WP_000679823.1	esterase	-
FZ043_RS17730	3546964..3548979	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
FZ043_RS17735	3548994..3549857	-	864	WP_149509010.1	neutral zinc metallopeptidase	-
FZ043_RS17740	3550004..3550402	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FZ043_RS17745	3550402..3550629	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FZ043_RS17750	3550783..3551496	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FZ043_RS17755	3551709..3552743	-	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
FZ043_RS17760	3552760..3553638	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FZ043_RS17765	3553784..3554356	+	573	WP_000176191.1	glycine cleavage system transcriptional repressor	-
FZ043_RS17770	3554356..3554826	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T138820 WP_000911330.1 NZ_CP043478:c3550402-3550004 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T138820 NZ_CP058236:c644912-644499 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT138820 WP_000450524.1 NZ_CP043478:c3550629-3550402 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT138820 NZ_CP058236:c645175-644909 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33