Detailed information of TA system TA138808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1142012..1142849
• Replicon: chromosome
• Accession: NZ_CP043478
• Organism: Escherichia coli strain ST130

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: FZ043_RS05310
• Protein ID: WP_000227784.1
• Coordinates: 1142012..1142554 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: FZ043_RS05315
• Protein ID: WP_001297137.1
• Coordinates: 1142538..1142849 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZ043_RS05290	1137551..1138462	-	912	WP_000705853.1	2-dehydropantoate 2-reductase	-
FZ043_RS05295	1138630..1139121	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
FZ043_RS05300	1139249..1140613	-	1365	WP_001000978.1	MFS transporter	-
FZ043_RS05305	1141021..1141956	+	936	WP_001297127.1	sel1 repeat family protein	-
FZ043_RS05310	1142012..1142554	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
FZ043_RS05315	1142538..1142849	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
FZ043_RS05320	1143034..1143924	-	891	WP_000971336.1	heme o synthase	-
FZ043_RS05325	1143936..1144265	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
FZ043_RS05330	1144265..1144879	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
FZ043_RS05335	1144869..1146860	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
FZ043_RS05340	1146882..1147829	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T138808 WP_000227784.1 NZ_CP043478:c1142554-1142012 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T138808 NZ_CP058228:c6193-5888 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT138808 WP_001297137.1 NZ_CP043478:c1142849-1142538 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT138808 NZ_CP058228:c6413-6195 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3