Detailed information of TA system TA138785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 93716..94281
• Replicon: plasmid unnamed2
• Accession: NZ_CP043439
• Organism: Cupriavidus campinensis strain MJ1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FZF10_RS00585
• Protein ID: WP_149135100.1
• Coordinates: 93910..94281 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FZF10_RS00580
• Protein ID: WP_149135099.1
• Coordinates: 93716..93913 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZF10_RS00550	90192..90410	+	219	WP_149135094.1	hypothetical protein	-
FZF10_RS00555	90515..90949	-	435	WP_149135095.1	hypothetical protein	-
FZF10_RS00560	90966..91157	-	192	WP_149135096.1	hypothetical protein	-
FZF10_RS00565	91291..91869	-	579	WP_149135097.1	hypothetical protein	-
FZF10_RS00570	91879..92828	-	950	Protein_114	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
FZF10_RS00575	92825..93550	-	726	WP_149135098.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
FZF10_RS00580	93716..93913	+	198	WP_149135099.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FZF10_RS00585	93910..94281	+	372	WP_149135100.1	type II toxin-antitoxin system VapC family toxin	Toxin
FZF10_RS00590	94360..94641	+	282	WP_149135101.1	hypothetical protein	-
FZF10_RS00595	94671..94889	+	219	Protein_119	hypothetical protein	-
FZF10_RS31195	95027..96601	+	1575	WP_187645074.1	hypothetical protein	-
FZF10_RS00605	96722..97054	+	333	WP_111733968.1	hypothetical protein	-
FZF10_RS00610	97084..97587	+	504	WP_111733969.1	hypothetical protein	-
FZF10_RS00615	97931..98293	-	363	WP_149135102.1	hypothetical protein	-
FZF10_RS00620	98353..98970	-	618	WP_149135103.1	DUF1851 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY	1..347552	347552

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-44)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13556.78 Da
• Isoelectric Point: 5.1296

>T138785 WP_149135100.1 NZ_CP043439:93910-94281 [Cupriavidus campinensis]
MILVDTSVWIDHINASDPMLISLLTQERVLIHPYVIGEISLGSLRDRDVVLGALRDLPGAPVATPEETLYLIEREGLFNR
GIGYVDTSLLASARLQPGIAIWTRDKRLKKVADELCLGAVLSH

Nucleotide

• Length: 372 bp

>T138785 NZ_CP058223:3585778-3587100 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAATGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAACCGTTATGCCAGACCGTTATCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTGCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTGTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCAGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTTCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACTGTCGCTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7105.10 Da
• Isoelectric Point: 8.6604

>AT138785 WP_149135099.1 NZ_CP043439:93716-93913 [Cupriavidus campinensis]
MRTTIALDDELLAKAQAYTGLEEKTALVREALKALIQREAAKRLANLGGSQPGIEGAPRRRQDTE

Nucleotide

• Length: 198 bp

>AT138785 NZ_CP043439:93716-93913 [Cupriavidus campinensis]
ATGCGTACGACCATTGCGCTTGACGATGAACTGTTGGCCAAGGCCCAAGCCTATACGGGGCTGGAAGAGAAAACGGCACT
TGTGCGCGAGGCGCTAAAGGCCTTGATCCAGCGTGAAGCCGCCAAACGACTCGCGAACCTTGGCGGGAGCCAACCGGGCA
TTGAAGGGGCACCGCGTCGCCGGCAGGACACCGAATGA