Detailed information of TA system TA138772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): KacT-ataR/DUF1778(antitoxin)
• Location: 1680309..1681106
• Replicon: chromosome
• Accession: NZ_CP043438
• Organism: Enterobacter sp. LU1

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A222RDD1
• Locus tag: FZF21_RS08000
• Protein ID: WP_032647926.1
• Coordinates: 1680585..1681106 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A331QJY7
• Locus tag: FZF21_RS07995
• Protein ID: WP_015570876.1
• Coordinates: 1680309..1680578 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZF21_RS07960	1675849..1676208	+	360	WP_017384779.1	helix-turn-helix transcriptional regulator	-
FZF21_RS07965	1676218..1676382	-	165	WP_017693668.1	helix-turn-helix transcriptional regulator	-
FZF21_RS07970	1676535..1677776	+	1242	WP_045333952.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
FZF21_RS07975	1677809..1678036	-	228	WP_006809324.1	YccJ family protein	-
FZF21_RS07980	1678055..1678651	-	597	WP_006809323.1	NAD(P)H:quinone oxidoreductase	-
FZF21_RS07985	1679043..1679213	+	171	WP_001273664.1	general stress protein	-
FZF21_RS07990	1679330..1680235	+	906	WP_032653183.1	DMT family transporter	-
FZF21_RS07995	1680309..1680578	+	270	WP_015570876.1	DUF1778 domain-containing protein	Antitoxin
FZF21_RS08000	1680585..1681106	+	522	WP_032647926.1	GNAT family N-acetyltransferase	Toxin
FZF21_RS08005	1681165..1682487	-	1323	WP_032670921.1	pyrimidine utilization transport protein G	-
FZF21_RS08010	1682509..1683000	-	492	WP_015570873.1	pyrimidine utilization flavin reductase protein F	-
FZF21_RS08015	1683013..1683603	-	591	WP_062934089.1	malonic semialdehyde reductase	-
FZF21_RS08020	1683613..1684413	-	801	WP_023296287.1	pyrimidine utilization protein D	-
FZF21_RS08025	1684421..1684807	-	387	WP_015570870.1	pyrimidine utilization protein C	-
FZF21_RS08030	1684819..1685508	-	690	WP_023296288.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-88)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19451.21 Da
• Isoelectric Point: 7.4318

>T138772 WP_032647926.1 NZ_CP043438:1680585-1681106 [Enterobacter sp. LU1]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLIRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Nucleotide

• Length: 522 bp

>T138772 NZ_CP058222:c4712689-4712516 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10247.75 Da
• Isoelectric Point: 5.8836

>AT138772 WP_015570876.1 NZ_CP043438:1680309-1680578 [Enterobacter sp. LU1]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWIAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT138772 NZ_CP058222:c4713013-4712717 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A222RDD1

Antitoxin

• AlphaFold DB: A0A331QJY7