Detailed information of TA system TA138752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1261480..1262397
• Replicon: chromosome
• Accession: NZ_CP043416
• Organism: Bacillus velezensis strain ONU 553

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: FZE25_RS06790
• Protein ID: WP_007407256.1
• Coordinates: 1261651..1262397 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: FZE25_RS06785
• Protein ID: WP_003154807.1
• Coordinates: 1261480..1261650 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZE25_RS06750	1256714..1258336	+	1623	WP_042635123.1	pyocin knob domain-containing protein	-
FZE25_RS06755	1258349..1258720	+	372	WP_007407260.1	hypothetical protein	-
FZE25_RS06760	1258725..1258922	+	198	WP_007610833.1	XkdX family protein	-
FZE25_RS06765	1258979..1259740	+	762	WP_007407258.1	hypothetical protein	-
FZE25_RS06770	1259792..1260055	+	264	WP_003154815.1	hemolysin XhlA family protein	-
FZE25_RS06775	1260069..1260332	+	264	WP_003154813.1	phage holin	-
FZE25_RS06780	1260346..1261224	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
FZE25_RS06785	1261480..1261650	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
FZE25_RS06790	1261651..1262397	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
FZE25_RS06795	1262502..1263500	-	999	WP_020955696.1	inorganic phosphate transporter	-
FZE25_RS06800	1263513..1264130	-	618	WP_003154804.1	DUF47 domain-containing protein	-
FZE25_RS06805	1264416..1265732	-	1317	WP_007407254.1	amino acid permease	-
FZE25_RS06810	1266055..1267005	+	951	WP_007407253.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1227843..1319705	91862

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T138752 WP_007407256.1 NZ_CP043416:c1262397-1261651 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T138752 NZ_CP058220:c4915027-4914716 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT138752 WP_003154807.1 NZ_CP043416:c1261650-1261480 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT138752 NZ_CP058220:c4914715-4914425 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14