Detailed information of TA system TA138751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 481647..482284
• Replicon: chromosome
• Accession: NZ_CP043416
• Organism: Bacillus velezensis strain ONU 553

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: FZE25_RS02485
• Protein ID: WP_003156187.1
• Coordinates: 481934..482284 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: FZE25_RS02480
• Protein ID: WP_003156188.1
• Coordinates: 481647..481928 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZE25_RS02460	478012..478611	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
FZE25_RS02465	478704..479069	+	366	WP_007410229.1	holo-ACP synthase	-
FZE25_RS02470	479234..480241	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
FZE25_RS02475	480358..481527	+	1170	WP_032866888.1	alanine racemase	-
FZE25_RS02480	481647..481928	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
FZE25_RS02485	481934..482284	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
FZE25_RS02490	482402..483223	+	822	WP_003156182.1	STAS domain-containing protein	-
FZE25_RS02495	483228..483593	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
FZE25_RS02500	483596..483997	+	402	WP_149061575.1	anti-sigma regulatory factor	-
FZE25_RS02505	484009..485016	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
FZE25_RS02510	485080..485409	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
FZE25_RS02515	485406..485888	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
FZE25_RS02520	485854..486642	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
FZE25_RS02525	486642..487244	+	603	Protein_451	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T138751 WP_003156187.1 NZ_CP043416:481934-482284 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T138751 NZ_CP043416:481934-482284 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT138751 WP_003156188.1 NZ_CP043416:481647-481928 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT138751 NZ_CP058220:c4607642-4607346 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5