Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3813227..3813845 Replicon chromosome
Accession NZ_CP043414
Organism Escherichia coli strain EC42405

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FZN31_RS18195 Protein ID WP_001291435.1
Coordinates 3813627..3813845 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FZN31_RS18190 Protein ID WP_000344800.1
Coordinates 3813227..3813601 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZN31_RS18180 3808315..3809508 + 1194 WP_001353399.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FZN31_RS18185 3809531..3812680 + 3150 WP_161399882.1 efflux RND transporter permease AcrB -
FZN31_RS18190 3813227..3813601 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FZN31_RS18195 3813627..3813845 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FZN31_RS18200 3814017..3814568 + 552 WP_105277205.1 maltose O-acetyltransferase -
FZN31_RS18205 3814684..3815154 + 471 WP_000136192.1 YlaC family protein -
FZN31_RS18210 3815318..3816868 + 1551 WP_001353400.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FZN31_RS18215 3816910..3817263 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FZN31_RS18225 3817642..3817953 + 312 WP_000409907.1 MGMT family protein -
FZN31_RS18230 3817984..3818556 - 573 WP_021566079.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T138738 WP_001291435.1 NZ_CP043414:3813627-3813845 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T138738 NZ_CP058220:1904016-1904267 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT138738 WP_000344800.1 NZ_CP043414:3813227-3813601 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT138738 NZ_CP043414:3813227-3813601 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGATTGCCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAAAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References