Detailed information of TA system TA138731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2573081..2574669
• Replicon: chromosome
• Accession: NZ_CP043414
• Organism: Escherichia coli strain EC42405

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: E9XK91
• Locus tag: FZN31_RS12365
• Protein ID: WP_001125414.1
• Coordinates: 2573347..2574669 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: FZN31_RS12360
• Protein ID: WP_001296726.1
• Coordinates: 2573081..2573347 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZN31_RS12360	2573081..2573347	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
FZN31_RS12365	2573347..2574669	+	1323	WP_001125414.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
FZN31_RS12370	2574868..2576220	-	1353	WP_001544475.1	SIR2 family protein	-
FZN31_RS12375	2576427..2577827	-	1401	WP_161399942.1	ISNCY family transposase	-
FZN31_RS12380	2578058..2578417	+	360	WP_161399941.1	hypothetical protein	-
FZN31_RS12385	2578559..2578753	-	195	WP_000956368.1	MbcA/ParS/Xre antitoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49388.08 Da
• Isoelectric Point: 8.8722

>T138731 WP_001125414.1 NZ_CP043414:2573347-2574669 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSKQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T138731 NZ_CP058219:c3088-2804 [Escherichia coli Nissle 1917]
ATGAGCTATAACCTTGAATTTGATCCCCGAGCCCTGAAGGAATGGCGCAAGCTCGGGGATGATGTCCGTCTGCAGTTCAA
GAAAAAACTCGAGCAGGTTCTACAACACCCGCGGATCGATAAAAATCGCCTGCGAGAGCTGCATGACTGCTACAAAATCA
AGCTCCGTGCATCCGGTTATCGCTTGGTCTATCAGGTTCGCGATCAAACCATTACGGTATTCGTGGTGGCGGTCGGTAAG
CGCGAGCGTTCTGCCGCTTACGATGCGGCCGATAAACGCTTATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT138731 WP_001296726.1 NZ_CP043414:2573081-2573347 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT138731 NZ_CP058219:c3320-3078 [Escherichia coli Nissle 1917]
ATGGCCACGATTAATATTCGGATCGATGACGAGCTGAAAAGCCGCTCTTATGCCGCACTGGAAAAGCTGGGCGTAACGCC
GTCCGAGGTTCTGCGCCAAACACTGGAATATGTGGCCCAAAGCGGACGTTTGCCGTTCCAGCAGGTTTTGCTGACCGAGG
ATGATGCCGATTTGATGGCTATCGTTCGGGATCGTCTGGAAAACCCACAGGCGGGCGTAAAGGTGTCACTGGATGAGCTA
TAA

Structures

Toxin

• AlphaFold DB: A0A1Q6BFU4