Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 981165..981819 Replicon chromosome
Accession NZ_CP043414
Organism Escherichia coli strain EC42405

Toxin (Protein)


Gene name cptA Uniprot ID F0JPE9
Locus tag FZN31_RS04720 Protein ID WP_000244774.1
Coordinates 981412..981819 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FZN31_RS04715 Protein ID WP_000354046.1
Coordinates 981165..981431 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZN31_RS04690 976326..977069 + 744 WP_000150658.1 SDR family oxidoreductase -
FZN31_RS04695 977126..978559 - 1434 WP_157944747.1 6-phospho-beta-glucosidase BglA -
FZN31_RS04700 978604..978915 + 312 WP_001182952.1 N(4)-acetylcytidine aminohydrolase -
FZN31_RS04705 979079..979738 + 660 WP_000250274.1 hemolysin III family protein -
FZN31_RS04710 979933..980913 - 981 WP_032171975.1 tRNA-modifying protein YgfZ -
FZN31_RS04715 981165..981431 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FZN31_RS04720 981412..981819 + 408 WP_000244774.1 protein YgfX Toxin
FZN31_RS04725 981859..982380 - 522 WP_001055865.1 flavodoxin FldB -
FZN31_RS04730 982492..983388 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FZN31_RS04735 983413..984123 + 711 WP_105276710.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FZN31_RS04740 984129..985862 + 1734 WP_105276709.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16078.05 Da        Isoelectric Point: 11.2511

>T138718 WP_000244774.1 NZ_CP043414:981412-981819 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T138718 NZ_CP043414:981412-981819 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGTTAAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCCCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCGCCGTGGATGATTAAAAGCGGCATGATGTTGCGTTTACGTTCTGATAGCGGTAAACGTCA
GCACTTGTGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT138718 WP_000354046.1 NZ_CP043414:981165-981431 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT138718 NZ_CP043414:981165-981431 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGTGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L6ZX35

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References