Detailed information of TA system TA138716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 229896..230508
• Replicon: chromosome
• Accession: NZ_CP043414
• Organism: Escherichia coli strain EC42405

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: FZN31_RS01060
• Protein ID: WP_000833473.1
• Coordinates: 229896..230081 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: FZN31_RS01065
• Protein ID: WP_000499744.1
• Coordinates: 230098..230508 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZN31_RS01050	226728..227879	+	1152	WP_000741504.1	L-threonine dehydrogenase	-
FZN31_RS01055	228080..229618	+	1539	WP_000183978.1	aldehyde dehydrogenase AldB	-
FZN31_RS01060	229896..230081	+	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
FZN31_RS01065	230098..230508	+	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FZN31_RS01070	230505..230819	-	315	WP_001064341.1	hypothetical protein	-
FZN31_RS01075	231002..231325	+	324	WP_000478193.1	DUF3302 domain-containing protein	-
FZN31_RS01080	231331..232467	+	1137	WP_105288438.1	HlyD family secretion protein	-
FZN31_RS01085	232464..233438	-	975	WP_105288439.1	LysR family transcriptional regulator	-
FZN31_RS01090	233562..234302	+	741	WP_000906829.1	MipA/OmpV family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T138716 WP_000833473.1 NZ_CP043414:229896-230081 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T138716 NZ_CP058217:1905461-1905715 [Escherichia coli Nissle 1917]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT138716 WP_000499744.1 NZ_CP043414:230098-230508 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT138716 NZ_CP058217:1905213-1905464 [Escherichia coli Nissle 1917]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9