138710

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 113220..113821 Replicon plasmid pNMBU-W13E19_01
Accession NZ_CP043407
Organism Escherichia coli strain NMBU-W13E19

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag FZN32_RS25405 Protein ID WP_001216034.1
Coordinates 113441..113821 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag FZN32_RS25400 Protein ID WP_001190712.1
Coordinates 113220..113441 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZN32_RS25375 108358..108576 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
FZN32_RS25380 109284..110279 + 996 WP_000246636.1 hypothetical protein -
FZN32_RS25385 110283..111215 + 933 WP_000991832.1 hypothetical protein -
FZN32_RS25390 111352..112049 - 698 Protein_140 IS1 family transposase -
FZN32_RS25395 112105..113037 - 933 Protein_141 type I restriction-modification system subunit M -
FZN32_RS25400 113220..113441 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FZN32_RS25405 113441..113821 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
FZN32_RS25410 113826..114005 + 180 WP_001513661.1 hypothetical protein -
FZN32_RS25415 114033..114392 + 360 WP_001513660.1 hypothetical protein -
FZN32_RS25420 114316..114729 + 414 Protein_146 DDE-type integrase/transposase/recombinase -
FZN32_RS25425 114679..114996 - 318 WP_001513659.1 hypothetical protein -
FZN32_RS25430 115224..116240 - 1017 WP_142975731.1 IS5-like element IS5 family transposase -
FZN32_RS25435 116448..117851 + 1404 WP_001373486.1 S-methylmethionine permease -
FZN32_RS25440 117838..118770 + 933 WP_023143441.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / dfrA14 / mph(A) - 1..122112 122112
- inside IScluster/Tn - - 111352..119601 8249


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T138710 WP_001216034.1 NZ_CP043407:113441-113821 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T138710 NZ_CP043407:113441-113821 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT138710 WP_001190712.1 NZ_CP043407:113220-113441 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT138710 NZ_CP058217:c197006-196943 [Escherichia coli Nissle 1917]
GTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References