138709

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 108051..108576 Replicon plasmid pNMBU-W13E19_01
Accession NZ_CP043407
Organism Escherichia coli strain NMBU-W13E19

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag FZN32_RS25370 Protein ID WP_001159868.1
Coordinates 108051..108356 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FZN32_RS25375 Protein ID WP_000813634.1
Coordinates 108358..108576 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZN32_RS25355 103961..105127 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
FZN32_RS25360 105715..106470 - 756 WP_000852146.1 replication initiation protein RepE -
FZN32_RS25365 107244..108050 - 807 WP_000016982.1 site-specific integrase -
FZN32_RS25370 108051..108356 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
FZN32_RS25375 108358..108576 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FZN32_RS25380 109284..110279 + 996 WP_000246636.1 hypothetical protein -
FZN32_RS25385 110283..111215 + 933 WP_000991832.1 hypothetical protein -
FZN32_RS25390 111352..112049 - 698 Protein_140 IS1 family transposase -
FZN32_RS25395 112105..113037 - 933 Protein_141 type I restriction-modification system subunit M -
FZN32_RS25400 113220..113441 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / dfrA14 / mph(A) - 1..122112 122112
- inside IScluster/Tn - - 111352..119601 8249


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T138709 WP_001159868.1 NZ_CP043407:c108356-108051 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T138709 NZ_CP058217:c47349-46924 [Escherichia coli Nissle 1917]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCTCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCAATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGGTCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCGACCGGCCTGCGGATTCAGTG
CAAAAACACGAATATAATCAGCCGGATGGCTGATTCAAGACATGATAAAAGACTTTACCAGCGCACGTTTAAAGCGTGCA
AGAGATGTTATTATACTAAAACATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT138709 WP_000813634.1 NZ_CP043407:c108576-108358 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT138709 NZ_CP058217:c47715-47380 [Escherichia coli Nissle 1917]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGTTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References