Detailed information of TA system TA138700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 5213..5772
• Replicon: plasmid pNMBU-W13E19_01
• Accession: NZ_CP043407
• Organism: Escherichia coli strain NMBU-W13E19

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: FZN32_RS24725
• Protein ID: WP_000421272.1
• Coordinates: 5213..5488 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: FZN32_RS24730
• Protein ID: WP_001387467.1
• Coordinates: 5488..5772 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZN32_RS24695	1263..2003	-	741	WP_001066953.1	tyrosine-type recombinase/integrase	-
FZN32_RS24700	2124..2306	-	183	WP_042344623.1	hypothetical protein	-
FZN32_RS24705	2649..2956	-	308	Protein_3	type II toxin-antitoxin system ParD family antitoxin	-
FZN32_RS24710	3449..4618	+	1170	WP_001072355.1	hypothetical protein	-
FZN32_RS24715	4651..4779	+	129	Protein_5	IS481 family transposase	-
FZN32_RS24720	4814..5107	-	294	WP_001105060.1	hypothetical protein	-
FZN32_RS24725	5213..5488	-	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FZN32_RS24730	5488..5772	-	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
FZN32_RS24735	6377..7129	+	753	WP_000562172.1	IclR family transcriptional regulator	-
FZN32_RS24740	7175..8140	-	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
FZN32_RS24745	8173..8553	-	381	WP_000710783.1	Rid family detoxifying hydrolase	-
FZN32_RS24750	8578..9228	-	651	Protein_12	dihydrodipicolinate synthase family protein	-
FZN32_RS24755	9294..9998	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
FZN32_RS24760	10056..10586	-	531	Protein_14	histidine-type phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / dfrA14 / mph(A)	-	1..122112	122112
	-	flank	IS/Tn	-	-	9294..9998	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T138700 WP_000421272.1 NZ_CP043407:c5488-5213 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T138700 NZ_CP058212:2080865-2081197 [Klebsiella michiganensis]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCAGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGCGGAAATTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCAGGGATAAAAACGTTGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGACTGCCCGGAACGGCAAGCGGCTGGAACGCATACCAGACGCTGTGGTAAATGAAGTGCTTGCCAGGCTGGATGC
AATTTTGAGTTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT138700 WP_001387467.1 NZ_CP043407:c5772-5488 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT138700 NZ_CP043407:c5772-5488 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880