Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4111103..4111904 Replicon chromosome
Accession NZ_CP043406
Organism Escherichia coli strain NMBU-W13E19

Toxin (Protein)


Gene name yeeV Uniprot ID D3GUT5
Locus tag FZN32_RS19960 Protein ID WP_001094430.1
Coordinates 4111103..4111480 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D3GUT4
Locus tag FZN32_RS19965 Protein ID WP_001285620.1
Coordinates 4111527..4111904 (-) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZN32_RS19935 4107234..4108217 - 984 WP_001329577.1 KpsF/GutQ family sugar-phosphate isomerase -
FZN32_RS19940 4109028..4109198 - 171 Protein_3890 IS110 family transposase -
FZN32_RS19945 4109541..4110383 - 843 WP_001532553.1 DUF4942 domain-containing protein -
FZN32_RS19950 4110468..4110665 - 198 WP_001374283.1 DUF957 domain-containing protein -
FZN32_RS19955 4110677..4111106 - 430 Protein_3893 hypothetical protein -
FZN32_RS19960 4111103..4111480 - 378 WP_001094430.1 TA system toxin CbtA family protein Toxin
FZN32_RS19965 4111527..4111904 - 378 WP_001285620.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
FZN32_RS19970 4112067..4112288 - 222 WP_000692329.1 DUF987 domain-containing protein -
FZN32_RS19975 4112351..4112827 - 477 WP_001186726.1 RadC family protein -
FZN32_RS19980 4112843..4113328 - 486 Protein_3898 antirestriction protein -
FZN32_RS19985 4113383..4114201 - 819 WP_001234620.1 DUF945 domain-containing protein -
FZN32_RS19990 4114301..4114534 - 234 WP_001119729.1 DUF905 family protein -
FZN32_RS19995 4114613..4115068 - 456 WP_000581504.1 hypothetical protein -
FZN32_RS20000 4115331..4115804 - 474 Protein_3902 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - kpsD / kpsE / kpsF / papH / papC / papD / papJ / papK / papE / papF / papX 4104314..4149003 44689


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13971.82 Da        Isoelectric Point: 6.8608

>T138698 WP_001094430.1 NZ_CP043406:c4111480-4111103 [Escherichia coli]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T138698 NZ_CP058212:c58648-58340 [Klebsiella michiganensis]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTGAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13698.52 Da        Isoelectric Point: 5.9505

>AT138698 WP_001285620.1 NZ_CP043406:c4111904-4111527 [Escherichia coli]
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Download         Length: 378 bp

>AT138698 NZ_CP058212:c58887-58651 [Klebsiella michiganensis]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y6M7

Antitoxin

Source ID Structure
PDB 2INW
AlphaFold DB A0A0E0Y522

References