Detailed information of TA system TA138698

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4111103..4111904
• Replicon: chromosome
• Accession: NZ_CP043406
• Organism: Escherichia coli strain NMBU-W13E19

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: D3GUT5
• Locus tag: FZN32_RS19960
• Protein ID: WP_001094430.1
• Coordinates: 4111103..4111480 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: D3GUT4
• Locus tag: FZN32_RS19965
• Protein ID: WP_001285620.1
• Coordinates: 4111527..4111904 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZN32_RS19935	4107234..4108217	-	984	WP_001329577.1	KpsF/GutQ family sugar-phosphate isomerase	-
FZN32_RS19940	4109028..4109198	-	171	Protein_3890	IS110 family transposase	-
FZN32_RS19945	4109541..4110383	-	843	WP_001532553.1	DUF4942 domain-containing protein	-
FZN32_RS19950	4110468..4110665	-	198	WP_001374283.1	DUF957 domain-containing protein	-
FZN32_RS19955	4110677..4111106	-	430	Protein_3893	hypothetical protein	-
FZN32_RS19960	4111103..4111480	-	378	WP_001094430.1	TA system toxin CbtA family protein	Toxin
FZN32_RS19965	4111527..4111904	-	378	WP_001285620.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
FZN32_RS19970	4112067..4112288	-	222	WP_000692329.1	DUF987 domain-containing protein	-
FZN32_RS19975	4112351..4112827	-	477	WP_001186726.1	RadC family protein	-
FZN32_RS19980	4112843..4113328	-	486	Protein_3898	antirestriction protein	-
FZN32_RS19985	4113383..4114201	-	819	WP_001234620.1	DUF945 domain-containing protein	-
FZN32_RS19990	4114301..4114534	-	234	WP_001119729.1	DUF905 family protein	-
FZN32_RS19995	4114613..4115068	-	456	WP_000581504.1	hypothetical protein	-
FZN32_RS20000	4115331..4115804	-	474	Protein_3902	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	kpsD / kpsE / kpsF / papH / papC / papD / papJ / papK / papE / papF / papX	4104314..4149003	44689

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13971.82 Da
• Isoelectric Point: 6.8608

>T138698 WP_001094430.1 NZ_CP043406:c4111480-4111103 [Escherichia coli]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T138698 NZ_CP058212:c58648-58340 [Klebsiella michiganensis]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTGAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13698.52 Da
• Isoelectric Point: 5.9505

>AT138698 WP_001285620.1 NZ_CP043406:c4111904-4111527 [Escherichia coli]
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Nucleotide

• Length: 378 bp

>AT138698 NZ_CP058212:c58887-58651 [Klebsiella michiganensis]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y6M7

Antitoxin

• AlphaFold DB: A0A0E0Y522