Detailed information of TA system TA138694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3458626..3459238
• Replicon: chromosome
• Accession: NZ_CP043406
• Organism: Escherichia coli strain NMBU-W13E19

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: FZN32_RS16750
• Protein ID: WP_000833473.1
• Coordinates: 3458626..3458811 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: FZN32_RS16755
• Protein ID: WP_000499744.1
• Coordinates: 3458828..3459238 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZN32_RS16740	3455458..3456609	+	1152	WP_000741501.1	L-threonine dehydrogenase	-
FZN32_RS16745	3456810..3458348	+	1539	WP_001309845.1	aldehyde dehydrogenase AldB	-
FZN32_RS16750	3458626..3458811	+	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
FZN32_RS16755	3458828..3459238	+	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FZN32_RS16760	3459310..3461274	-	1965	WP_001026861.1	glycoside hydrolase family 127 protein	-
FZN32_RS16765	3461285..3462685	-	1401	WP_000204802.1	MFS transporter	-
FZN32_RS16770	3462911..3463726	+	816	WP_000891825.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T138694 WP_000833473.1 NZ_CP043406:3458626-3458811 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T138694 NZ_CP058211:c8198-7893 [Escherichia marmotae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCACCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGGGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT138694 WP_000499744.1 NZ_CP043406:3458828-3459238 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT138694 NZ_CP058211:c8418-8200 [Escherichia marmotae]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATAAGTTACTCAAAGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9