Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2832036..2832533 Replicon chromosome
Accession NZ_CP043406
Organism Escherichia coli strain NMBU-W13E19

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FZN32_RS13940 Protein ID WP_001173343.1
Coordinates 2832036..2832209 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FZN32_RS13945 Protein ID WP_000398619.1
Coordinates 2832237..2832533 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZN32_RS13930 2828707..2830164 + 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -
FZN32_RS13935 2830401..2831918 + 1518 WP_000003806.1 lysine--tRNA ligase -
FZN32_RS13940 2832036..2832209 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FZN32_RS13945 2832237..2832533 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FZN32_RS13950 2832762..2833034 - 273 WP_000405647.1 N-acetyltransferase -
FZN32_RS13955 2833046..2833276 - 231 WP_000373291.1 (4Fe-4S)-binding protein -
FZN32_RS13960 2833457..2835088 + 1632 WP_001216477.1 sensor histidine kinase -
FZN32_RS13965 2835085..2835804 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T138693 WP_001173343.1 NZ_CP043406:c2832209-2832036 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T138693 NZ_CP058210:72918-73070 [Escherichia marmotae]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT138693 WP_000398619.1 NZ_CP043406:c2832533-2832237 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT138693 NZ_CP058210:c72863-72806 [Escherichia marmotae]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References